Molecular Cloning and Sexually Dimorphic Expression Analysis of nanos2 in the Sea Urchin, Mesocentrotus nudus
Molecular Cloning and Sexually Dimorphic Expression Analysis of nanos2 in the Sea Urchin, Mesocentrotus nudus
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海胆中海胆nanos2的分子克隆和两性二态性表达分析
DOI：
10.3390/ijms20112705
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发表时间：
2019-06
影响因子：
5.6
通讯作者：
Yaqing Chang
中科院分区：
文献类型：
--
作者：
Jian Zhang;Xiao Han;Jin Wang;Bingzheng Liu;Jinliang Wei;Weijie Zhang;Zhihui Sun;Yaqing Chang
裸露中海胆是中国重要的经济养殖物种，性腺是人类唯一可食用的部位。性腺发育的分子机制近年来受到越来越多的关注。虽然Nanos2基因已被确认为几种无脊椎动物的生殖细胞标记，但对成年海胆中的nanos2知之甚少。其中，我们报道了裸分支杆菌Nanos2同源基因Mnnan2的特征。Mnnanos2是一个母性因子，在胚胎发育和早期个体发育过程中可以连续检测到。实时定量聚合酶链式反应(RT-qPCR)和切片原位杂交(ISH)分析揭示了MnNa2在性腺中的动态和性别二态表达模式。随着卵巢和睾丸的性腺发育，其表达在第2期达到最高水平。在卵巢中，Mnnanos2在生殖细胞中特异表达。相反，Mnnanos2在睾丸的营养吞噬细胞(NP)和雄性生殖细胞中都有表达。此外，通过RNA干扰(RNAi)方法击倒Mnnanos2降低了Nanos2和BULEE的表达，但反过来却增加了FOXL2的表达。因此，我们的数据表明，Mnnanos2可能在配子发生过程中作为雌性生殖细胞标记，并为揭示其在成年海胆中的功能提供了机会。
Sea urchin (Mesocentrotus nudus) is an economically important mariculture species in China and the gonads are the solely edible parts to human. The molecular mechanisms of gonad development have attracted increasing attention in recent years. Although the nanos2 gene has been identified as a germ cell marker in several invertebrates, little is known about nanos2 in adult sea urchins. Hereinto, we report the characterization of Mnnano2, an M. nudus nanos2 homology gene. Mnnanos2 is a maternal factor and can be detected continuously during embryogenesis and early ontogeny. Real-time quantitative PCR (RT-qPCR) and section in situ hybridization (ISH) analysis revealed a dynamic and sexually dimorphic expression pattern of Mnnano2 in the gonads. Its expression reached the maximal level at Stage 2 along with the gonad development in both ovary and testis. In the ovary, Mnnanos2 is specifically expressed in germ cells. In contrast, Mnnanos2 is expressed in both nutritive phagocytes (NP) cells and male germ cells in testis. Moreover, knocking down of Mnnanos2 by means of RNA interference (RNAi) reduced nanos2 and boule expression but conversely increased the expression of foxl2. Therefore, our data suggest that Mnnanos2 may serve as a female germ cell marker during gametogenesis and provide chances to uncover its function in adult sea urchin.
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Oulhen N;Wessel GM
通讯作者：
Wessel GM
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作者：
K. Subramaniam;G. Seydoux
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Charlotte I. Wang;R. Lehmann
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