Early Evolution of Vertebrate Mybs: An Integrative Perspective Combining Synteny, Phylogenetic, and Gene Expression Analyses.
Early Evolution of Vertebrate Mybs: An Integrative Perspective Combining Synteny, Phylogenetic, and Gene Expression Analyses.
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DOI：
10.1093/gbe/evv197
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发表时间：
2015-10-15
影响因子：
3.3
通讯作者：
Hoffmann FG
中科院分区：
文献类型：
--
作者：
Campanini EB;Vandewege MW;Pillai NE;Tay BH;Jones JL;Venkatesh B;Hoffmann FG
在大多数脊椎动物中，MyB超家族中的基因编码三种相关的转录因子，A-、B-和c-MyB，具有功能上不同的作用，而大多数无脊椎动物具有单个MyB。B-Myb在细胞分裂和细胞周期进程中起重要作用，c-Myb参与造血，A-Myb参与精子发生和调节粗线期PIWI相互作用RNA的表达，这是一类参与转录后基因调控和生殖组织维持的小RNA。硬骨鱼和四足动物之间的比较表明，Myb基因的出现和功能分歧与脊椎动物进化早期的两轮全基因组复制有关。我们结合系统发育，同线，结构和基因表达分析的Myb旁系同源的象鲨和七鳃鳗的数据从12个脊椎动物重建脊椎动物Mybs的早期进化。系统发育和同线性分析表明，象鲨和日本七鳃鳗有A-，B-和c-MyB基因的副本，这意味着它们的起源可以追溯到七鳃鳗和有颌鱼类的共同祖先。然而，结构和基因表达分析表明，它们的功能角色之间的分歧有颚类和圆口类。特别是，我们没有检测到A-Myb在睾丸中的表达，这表明A-Myb参与粗线期PIWI相互作用的RNA途径可能是一个颚口特异性的创新。我们推测，在七鳃鳗A-Myb的中央结构域的二次损失的cyclostome和gnathostome A-Myb蛋白质之间的功能差异的基础。
The genes in the Myb superfamily encode for three related transcription factors in most vertebrates, A-, B-, and c-Myb, with functionally distinct roles, whereas most invertebrates have a single Myb. B-Myb plays an essential role in cell division and cell cycle progression, c-Myb is involved in hematopoiesis, and A-Myb is involved in spermatogenesis and regulating expression of pachytene PIWI interacting RNAs, a class of small RNAs involved in posttranscriptional gene regulation and the maintenance of reproductive tissues. Comparisons between teleost fish and tetrapods suggest that the emergence and functional divergence of the Myb genes were linked to the two rounds of whole-genome duplication early in vertebrate evolution. We combined phylogenetic, synteny, structural, and gene expression analyses of the Myb paralogs from elephant shark and lampreys with data from 12 bony vertebrates to reconstruct the early evolution of vertebrate Mybs. Phylogenetic and synteny analyses suggest that the elephant shark and Japanese lamprey have copies of the A-, B-, and c-Myb genes, implying their origin could be traced back to the common ancestor of lampreys and gnathostomes. However, structural and gene expression analyses suggest that their functional roles diverged between gnathostomes and cyclostomes. In particular, we did not detect A-Myb expression in testis suggesting that the involvement of A-Myb in the pachytene PIWI interacting RNA pathway is probably a gnathostome-specific innovation. We speculate that the secondary loss of a central domain in lamprey A-Myb underlies the functional differences between the cyclostome and gnathostome A-Myb proteins.
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影响因子：
14.9
作者：
Letunic I;Doerks T;Bork P
通讯作者：
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2.3
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Guthrie, E
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14.9
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通讯作者：
Flicek P