Discovery and fine mapping of a novel resistance locus to Fusarium wilt race 2 in lettuce (Lactuca sativa L.)
Discovery and fine mapping of a novel resistance locus to Fusarium wilt race 2 in lettuce (Lactuca sativa L.)
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生菜 (Lactuca sativa L.) 中镰刀菌枯萎病小种 2 的新抗性位点的发现和精细定位
DOI：
10.1007/s10681-022-03058-0
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发表时间：
2022
期刊：
影响因子：
1.9
通讯作者：
T.
中科院分区：
文献类型：
--
作者：
Seki;K.;Shimizu;Y.;Hayashi;M.;Sassa;H.;Kikuchi;S.;Taguchi;A.;Chino;F.;Soga;E.;Serizawa;H. and Koba;T.
在全球变暖的影响下，热带和亚热带地区莴苣枯萎病的危害日益严重。在此之前，我们报道了在LG1上开发了一个基于pcr的标记LG1_v8_116.506Mbp，用于鉴定枯萎病2号小种的抗性。利用分子标记技术对莴苣2号小种的新抗性遗传资源进行了研究。比较48个品种/系的基因型和表型发现，最初被认为具有相同抗性基因的‘CGN17397’和‘2008-11’在LG1上表现出抗性表型，但在标记上表现出易感基因型。10个抗病品种/品系的等位基因试验表明，‘CGN17397’的抗性并未定位在LG1上。利用来自‘CGN17397’的2个f2群体，通过AFLP分析和pcr标记的精细定位，明确了该抗性是由位于LG7上63.476 ~ 66.012 Mbp区间的单个半显性基因控制的。在与抗性表型完全共分离的4个标记中，只有lg7_v8_65 .645 mbps允许我们通过对48个品种/系的基因分型来鉴定抗性基因型。根据“Salinas”参考基因组序列的注释，LG7上的抗性位点有12个抗病候选基因。阐明LG1和LG7两个基因座的潜在耐药机制是未来关注的问题。这些结果可能有助于加快培育抗枯萎病品种，并进一步了解莴苣抗2号小种的基本机制。
The influence of Fusarium wilt in lettuce (Lactuca sativaL.) is becoming increasingly severe under the influence of global warming in tropical and subtropical regions. Previously, we reported on the development of a PCR-based marker,LG1_v8_116.506Mbp, on LG1 for the resistance to Fusarium wilt race 2. This study investigated genetic resources with novel resistance to race 2 using molecular markers for stable lettuce production. Comparing genotypes and phenotypes of the 48 cultivars/lines, it was found that ‘CGN17397’ and ‘2008–11’, which were at first considered to possess the same resistance genes, showed resistant phenotypes but had susceptible genotypes in the marker on LG1. Allelism tests using ten resistant cultivars/lines showed that the resistance derived from ‘CGN17397’ was not located on LG1. Using the two F2populations derived from ‘CGN17397’, it was clarified that the resistance was controlled by a single semi-dominant gene located at the region between 63.476 Mbp and 66.012 Mbp on LG7, defined by AFLP analysis and fine mapping with PCR-based markers. Of the four markers which showed complete co-segregation with the resistance phenotype, onlyLG7_v8_65.645Mbpallowed us to identify the resistance genotype by genotyping the 48 cultivars/lines. According to the annotation of the reference genome sequence of ‘Salinas’, the locus for resistance on LG7 had twelve disease resistance gene candidates. The elucidation of underlying resistance mechanisms in the two loci on LG1 and LG7 is of future concern. These results may contribute to accelerating the breeding of Fusarium wilt-resistant cultivars and add understanding to the fundamental mechanisms for resistance to race 2 in lettuce.
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生菜品种“Celtuce”对尖孢镰刀菌小种 2 的抗性遗传。
DOI：
10.1270/jsbbr.11.155
发表时间：
2009
期刊：
Breeding Research
影响因子：
--
作者：
J. Shimazu;N. Yamauchi;T. Shirakawa
通讯作者：
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作者：
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通讯作者：
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影响因子：
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作者：
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通讯作者：
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影响因子：
1.2
作者：
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影响因子：
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作者：
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通讯作者：
M. Tsukada