An Assay for Systematically Quantifying the Vestibulo-Ocular Reflex to Assess Vestibular Function in Zebrafish Larvae.
An Assay for Systematically Quantifying the Vestibulo-Ocular Reflex to Assess Vestibular Function in Zebrafish Larvae.
复制标题
系统量化前庭眼反射以评估斑马鱼幼虫前庭功能的测定
DOI：
10.3389/fncel.2018.00257
复制
发表时间：
2018
影响因子：
5.3
通讯作者：
Chen F
中科院分区：
文献类型：
--
作者：
Sun P;Zhang Y;Zhao F;Wu JP;Pun SH;Peng C;Du M;Vai MI;Liu D;Chen F
斑马鱼（Danio rerio）幼虫被广泛用于研究耳功能，因为它们具有包括听觉和平衡在内的所有五种典型脊椎动物感觉。强大的遗传工具和透明的胚胎和幼体身体也使斑马鱼成为研究耳发育的独特脊椎动物模型。由于斑马鱼幼鱼体积小，实验过程中对水分的要求较高，因此准确获取斑马鱼幼鱼的前庭眼反射（VOR）是一项具有挑战性的工作。本研究开发了一种新的VOR测试装置，用于测量斑马鱼幼体头部绕地球水平轴运动引起的线性VOR。该系统有一个新设计的幼虫形腔室，活鱼可以稳定地保持在没有麻醉的情况下，该系统比以前的系统更紧凑，更容易使用。为了证明该系统的有效性，测量了野生型（WT）、dlx3b和dlx4b morphant斑马鱼幼鱼的LVOR，结果表明LVOR幅度与吗啉代寡核苷酸（MO）诱导的耳石形态学变化一致。我们的研究代表了一个重要的进展，以获得VOR和预测前庭条件的斑马鱼。
Zebrafish (Danio rerio) larvae are widely used to study otic functions because they possess all five typical vertebrate senses including hearing and balance. Powerful genetic tools and the transparent body of the embryo and larva also make zebrafish a unique vertebrate model to study otic development. Due to its small larval size and moisture requirement during experiments, accurately acquiring the vestibulo-ocular reflex (VOR) of zebrafish larva is challenging. In this report, a new VOR testing device has been developed for quantifying linear VOR (LVOR) in zebrafish larva, evoked by the head motion about the earth horizontal axis. The system has a newly designed larva-shaped chamber, by which live fish can be steadily held without anesthesia, and the system is more compact and easier to use than its predecessors. To demonstrate the efficacy of the system, the LVORs in wild-type (WT), dlx3b and dlx4b morphant zebrafish larvae were measured and the results showed that LVOR amplitudes were consistent with the morphological changes of otoliths induced by morpholino oligonucleotides (MO). Our study represents an important advance to obtain VOR and predict the vestibular conditions in zebrafish.
登录
查看更多内容
影响因子：
4.6
作者：
Inoue, Maya;Tanimoto, Masashi;Oda, Yoichi
通讯作者：
Oda, Yoichi
DOI：
10.3791/50832
发表时间：
2013-10-09
期刊：
Journal of visualized experiments : JoVE
影响因子：
--
作者：
Cameron DJ;Rassamdana F;Tam P;Dang K;Yanez C;Ghaemmaghami S;Dehkordi MI
通讯作者：
Dehkordi MI
影响因子：
3.4
作者：
Dai M;Cohen B;Smouha E;Cho C
通讯作者：
Cho C
影响因子：
1.4
作者：
CURTHOYS, IS
通讯作者：
CURTHOYS, IS
影响因子：
3
作者：
Bang, PI;Yelick, PC;Sewell, WF
通讯作者：
Sewell, WF