In vivo magnetic enrichment, photoacoustic diagnosis, and photothermal purging of infected blood using multifunctional gold and magnetic nanoparticles.
In vivo magnetic enrichment, photoacoustic diagnosis, and photothermal purging of infected blood using multifunctional gold and magnetic nanoparticles.
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DOI：
10.1371/journal.pone.0045557
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Zharov VP
中科院分区：
文献类型：
--
作者：
Galanzha EI;Shashkov E;Sarimollaoglu M;Beenken KE;Basnakian AG;Shirtliff ME;Kim JW;Smeltzer MS;Zharov VP
细菌感染是全世界发病率和死亡率的主要原因。由于脓毒性休克和转移性感染的可能性，菌血症是一个特别值得关注的问题。由于抗生素耐药菌株的出现，菌血症的治疗日益受到损害，因此迫切需要替代疗法。本文介绍了一种组织和血液中金黄色葡萄球菌的体内光声（PA）检测和光热（PT）根除方法。本研究表明，该方法可应用于内源性类胡萝卜素的内在近红外吸收，非线性PA和PT对比增强，在血液中进行无标签诊断和治疗。为了提高循环细菌细胞（CBCs）检测的敏感性和特异性，用抗体混合物功能化双色金和具有巨大PA和PT对比扩增的多层磁性纳米颗粒，用于分子靶向金黄色葡萄球菌表面相关标记物，如蛋白A和脂蛋白。通过小鼠模型，证明了该方法的实用性，可超灵敏地检测CBCs，阈值灵敏度低至0.5 CBCs/mL，体内磁富集CBCs， PT根除CBCs，并通过CBC计数实时监测治疗效果。我们的PA-PT纳米治疗平台集成了体内多重靶向、磁富集、信号放大、多色识别和反馈控制，可以作为一种生物学工具来深入了解CBCs的传播途径、细菌重新播撒的感染进展以及败血症的发生和治疗，并且在人类中可能是可行的，特别是使用旁路原理图。
Bacterial infections are a primary cause of morbidity and mortality worldwide. Bacteremia is a particular concern owing to the possibility of septic shock and the development of metastatic infections. Treatment of bacteremia is increasingly compromised by the emergence of antibiotic resistant strains, creating an urgent need for alternative therapy. Here, we introduce a method for in vivo photoacoustic (PA) detection and photothermal (PT) eradication of Staphylococcus aureus in tissue and blood. We show that this method could be applicable for label-free diagnosis and treatment of in the bloodstream using intrinsic near-infrared absorption of endogenous carotenoids with nonlinear PA and PT contrast enhancement. To improve sensitivity and specificity for detection of circulating bacteria cells (CBCs), two-color gold and multilayer magnetic nanoparticles with giant amplifications of PA and PT contrasts were functionalized with an antibody cocktail for molecular targeting of S. aureus surface-associated markers such as protein A and lipoprotein. With a murine model, the utility of this approach was demonstrated for ultrasensitive detection of CBCs with threshold sensitivity as low as 0.5 CBCs/mL, in vivo magnetic enrichment of CBCs, PT eradication of CBCs, and real-time monitoring of therapeutic efficacy by CBC counting. Our PA-PT nano-theranostic platform, which integrates in vivo multiplex targeting, magnetic enrichment, signal amplification, multicolor recognition, and feedback control, could be used as a biological tool to gain insights on dissemination pathways of CBCs, infection progression by bacteria re-seeding, and sepsis development and treatment, and could potentially be feasible in humans, especially using bypass schematic.
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DOI：
10.1186/bcr2561
发表时间：
2010
期刊：
Breast cancer research : BCR
影响因子：
--
作者：
Aguirre-Ghiso JA
通讯作者：
Aguirre-Ghiso JA
影响因子：
38.3
作者：
通讯作者：
--
影响因子：
6.4
作者：
Fowler, Vance G., Jr.;Nelson, Charlotte L.;Gill, Steven R.
通讯作者：
Gill, Steven R.
影响因子：
2.4
作者：
Kim, Jin-Woo;Shashkov, Evgeny V.;Zharov, Vladimir P.
通讯作者：
Zharov, Vladimir P.
DOI：
10.1073/pnas.2232479100
发表时间：
2003-11-11
影响因子：
11.1
作者：
Hirsch, LR;Stafford, RJ;West, JL
通讯作者：
West, JL