Purification of a water extract of Chinese sweet tea plant (Rubus suavissimus S. Lee) by alcohol precipitation.
Purification of a water extract of Chinese sweet tea plant (Rubus suavissimus S. Lee) by alcohol precipitation.
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DOI：
10.1021/jf900269r
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发表时间：
2009-06-10
影响因子：
6.1
通讯作者：
Liu Z
中科院分区：
文献类型：
--
作者：
Koh GY;Chou G;Liu Z
甜茶叶的水提过程中经常会产生大量的非活性多糖成分。为了纯化该水提取物以获得潜在的提高的生物活性，应用由梯度方案组成的醇沉淀（AP），并通过比色和HPLC分析来检查其结果。AP是有效的分配水粗提物成可溶性上清液和不溶性沉淀，其效果随酒精方案显着变化。通常，酒精浓度越高，所得提取物越纯。在其最大值下，约36%（w/w）的粗提取物（其中23%是多糖）被沉淀并去除，得到由超过20%的生物活性标记化合物（没食子酸、鞣花酸、芦丁、甜茶苷和甜菊醇单苷）组成的纯化提取物。在70%乙醇浓度下，醇沉法可完全除去粗提物中11%的多糖。更高的酒精含量导致更纯的提取物，可能是通过去除一些生物活性不确定的化合物。醇沉法是从甜茶水提液中去除多糖的有效方法，可作为许多含有大量多糖的水生植物提取液的初步简单纯化工具。
The aqueous extraction process of the leaves of Rubus suavissimus often brings in a large amount of non-active polysaccharides as part of the constituents. To purify this water extract for potential elevated bioactivity, alcohol precipitation (AP) consisting of gradient regimens was applied, and its resultants were examined through colorimetric and HPLC analyses. AP was effective in partitioning the aqueous crude extract into a soluble supernatant and an insoluble precipitant, and its effect varied significantly with alcohol regimens. Generally, the higher the alcohol concentration, the purer was the resultant extract. At its maximum, approximately 36% (w/w) of the crude extract, of which 23% was polysaccharides, was precipitated and removed, resulting in a purified extract consisting of over 20% bioactive marker compounds (gallic acid, ellagic acid, rutin, rubusoside, and steviol monoside). The removal of 11% polysaccharides from the crude water extract by using alcohol precipitation was complete at 70% alcohol regimen. Higher alcohol levels resulted in even purer extracts, possibly by removing some compounds of uncertain bioactivity. Alcohol precipitation is an effective way of removing polysaccharides from the water extract of sweet tea plant and could be used as an initial simple purification tool for many water plant extracts that contain large amounts of polysaccharides.
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