Sustained post-mating response in Drosophila melanogaster requires multiple seminal fluid proteins.
Sustained post-mating response in Drosophila melanogaster requires multiple seminal fluid proteins.
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果蝇中持续的交战反应需要多种精确的液体蛋白。
DOI：
10.1371/journal.pgen.0030238
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发表时间：
2007-12
期刊：
影响因子：
4.5
通讯作者：
Wolfner MF
中科院分区：
文献类型：
--
作者：
Ram KR;Wolfner MF
成功的繁殖是将基因传给下一代的关键。精液蛋白有助于重要的生殖过程，导致从昆虫到哺乳动物的各种物种的受精。在果蝇中，雄性的附腺是精液蛋白质的来源，这些蛋白质通过改变雌性交配后的行为和生理来影响雄性和雌性的生殖输出。果蝇精液中发现的蛋白质类别与包括哺乳动物在内的其他生物的蛋白质类别相似。通过RNA干扰(RNAi)下调个体附腺蛋白(ACPs)的水平，研究了25个ACPs在雌性交配后的3种反应中的作用：产卵、对生殖的接受性和精子储存。我们检测了五个ACP在这些交配后反应中的作用。在交配后的第一天，需要CG33943来充分刺激产卵。另外四个ACP(CG1652、CG1656、CG17575和CG9997)似乎调节交配后行为和生理变化的长期反应。交配后的长期反应需要储存精子，到目前为止，已知只需要单一的ACP。在这里，我们发现了几个新的ACP一起需要，这些共同需要持续的卵子产生，降低对交配雌性的接受性，以及促进储存的精子从精囊中释放。我们的结果还表明，从昆虫到哺乳动物的精浆中发现的保守蛋白质类成员对重要的生殖过程是必不可少的。在有性繁殖生物体中，精子与精液蛋白一起进入雌性体内，精液蛋白对生育能力至关重要。这些蛋白质可以激活精子或增强女性体内的精子存储，并可以提高精子与卵子受精的机会。了解精液蛋白的作用在害虫控制和某些人类不育症的诊断中具有潜在的实用价值。然而，很少有精液蛋白的确切功能是已知的。为了解决这个问题，我们分别在雄性果蝇中降低了25种精液蛋白的水平，并测试了雄性果蝇调节卵子产生、精子储存/释放或配偶行为的能力。我们发现了五种精液蛋白质，它们是提高交配雌性后代繁殖力所必需的。这些蛋白质中的四种是有效释放精子以使卵子受精所必需的，这一功能以前没有分配给任何精液蛋白。从昆虫到人类，这四种生物都在保存在精液中的生化类中，这表明它们在其他动物中可能扮演着类似的精子相关角色。除了将功能分配给特定的精液蛋白质外，我们的结果表明，果蝇可以作为一个模型，用来剖析精液中保守的蛋白质类别的功能。
Successful reproduction is critical to pass genes to the next generation. Seminal proteins contribute to important reproductive processes that lead to fertilization in species ranging from insects to mammals. In Drosophila, the male's accessory gland is a source of seminal fluid proteins that affect the reproductive output of males and females by altering female post-mating behavior and physiology. Protein classes found in the seminal fluid of Drosophila are similar to those of other organisms, including mammals. By using RNA interference (RNAi) to knock down levels of individual accessory gland proteins (Acps), we investigated the role of 25 Acps in mediating three post-mating female responses: egg production, receptivity to remating and storage of sperm. We detected roles for five Acps in these post-mating responses. CG33943 is required for full stimulation of egg production on the first day after mating. Four other Acps (CG1652, CG1656, CG17575, and CG9997) appear to modulate the long-term response, which is the maintenance of post-mating behavior and physiological changes. The long-term post-mating response requires presence of sperm in storage and, until now, had been known to require only a single Acp. Here, we discovered several novel Acps together are required which together are required for sustained egg production, reduction in receptivity to remating of the mated female and for promotion of stored sperm release from the seminal receptacle. Our results also show that members of conserved protein classes found in seminal plasma from insects to mammals are essential for important reproductive processes. In sexually reproducing organisms, sperm enter the female in combination with seminal proteins that are critical for fertility. These proteins can activate sperm or enhance sperm storage within the female, and can improve the chance that sperm will fertilize eggs. Understanding the action of seminal proteins has potential utility in insect pest control and in the diagnosis of certain human infertilities. However, the precise function of very few seminal proteins is known. To address this, we knocked down the levels of 25 seminal proteins individually in male fruit flies, and tested the males' abilities to modulate egg production, sperm storage/release, or behavior of their mates. We found five seminal proteins that are necessary to elevate offspring production in mated females. Four of these proteins are needed for efficient release of sperm from storage to fertilize eggs, a function that had not been previously assigned to any seminal protein. All four are in biochemical classes that are conserved in seminal fluid from insects to humans, suggesting they may play similar sperm-related roles in other animals. In addition to assigning functions to particular seminal proteins, our results suggest that fruit flies can serve as a model with which to dissect the functions of conserved protein classes in seminal fluid.
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DOI：
10.1073/pnas.92.22.10114
发表时间：
1995-10-24
影响因子：
11.1
作者：
HERNDON, LA;WOLFNER, MF
通讯作者：
WOLFNER, MF
男性对蜜蜂精子储存和交配的贡献的蛋白质组学分析。
影响因子：
2.6
作者：
Collins, A M;Caperna, T J;Williams, V;Garrett, W M;Evans, J D
通讯作者：
Evans, J D
影响因子：
9.2
作者：
Carvalho, GB;Kapahi, P;Benzer, S
通讯作者：
Benzer, S
影响因子：
4
作者：
He, SP;Lin, YL;Liu, YX
通讯作者：
Liu, YX
影响因子：
3.3
作者：
Fiumera, Anthony C.;Dumont, Bethany L.;Clark, Andrew G.
通讯作者：
Clark, Andrew G.