Self-propelled quantum dots-based spherical nucleic amplifier for ratiometric imaging of intracellular microRNAs
Self-propelled quantum dots-based spherical nucleic amplifier for ratiometric imaging of intracellular microRNAs
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基于自驱动量子点的球形核酸放大器，用于细胞内 microRNA 的比例成像
DOI：
10.1016/j.snb.2022.132802
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发表时间：
2022-10
期刊：
影响因子：
--
通讯作者：
Xiaoqing Chen
中科院分区：
文献类型：
--
作者：
Xinyi Zhao;Hai Wang;Yinghui Feng;Hexiang Li;Qi Liu;Xiaoqing Chen
活细胞中顶针肿瘤相关 microRNA (miRNA) 的实时原位可视化和灵敏检测尚未得到满足。特别是，较差的扩增效率和对复杂细胞内环境的抵抗力阻碍了放大器在体内的广泛应用。在此，我们报告了基于无酶自驱动量子点的球形核酸（基于 QD 的 SNA，也称为 TSD-QD）作为比例放大器，用于稳定、高效、灵敏地检测和成像生物样品和活细胞中的 miRNA。事实证明，比率放大器在人血清和细胞中具有出色的稳定性，并且对 miRNA 具有出色的识别能力。更重要的是，巧妙设计的燃料链（FS）尾部序列可以使TSD-QD在体外非酶检测中具有较高的运行效率和优异的检测性能（范围为10 fM-150 nM，检测限（LOD）为5.8 fM）。此外，球形核酸（SNA）结构可以赋予TSD-QD良好的光稳定性和对核酸酶的高抗性，从而有利于其通过荧光成像监测细胞内miRNA-21的应用。凭借这些优点，所提出的基于 TSD-QD 的放大器能够准确有效地监测活细胞（A549、正常 QSG-7701、Hela 和 MCF-7 细胞）中的 miRNA 表达水平，并在医学诊断和生物医学应用中具有巨大潜力。
Real-timein-situvisualization and sensitive detection of thimbleful tumor-related microRNAs (miRNAs) in living cells is still unmet. Especially, poor amplification efficiency and resistance against complex intracellular environment hinder amplifiers’ widespread applicationsin vivo. Herein, we report enzyme-free self-propelled quantum dots-based spherical nucleic acids (QDs-based SNAs, also named TSD-QDs) as a ratiometric amplifier for stable, efficient, and sensitive detection and imaging of miRNAs in biological samples and living cells. The ratiometric amplifier is proved possesses outstanding stability in human serum and cells, as well as prominent recognition capability for miRNAs. More importantly, the ingeniously designed tail sequence of fuel strand (FS) could lead to high operating efficiency and excellent detection performance of TSD-QDs for non-enzyme detectionin vitro(in the range of 10 fM-150 nM with a limit of detection (LOD) of 5.8 fM). Furthermore, the spherical nucleic acids (SNAs) structure can endow TSD-QDs with favorable photostability and high resistance against to the nuclease, thus benefit its applications in intracellular miRNA-21 monitoringviafluorescence imaging. With these advantages, the proposed TSD-QDs based amplifier enables accurate and effective monitoring of the miRNAs expression levels in living cells (A549, normal QSG-7701, Hela and MCF-7 cells), and poses great potential in medical diagnostics and biomedical applications.
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