Screening potential biomarkers for colorectal cancer based on circular RNA chips.
Screening potential biomarkers for colorectal cancer based on circular RNA chips.
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DOI：
10.3892/or.2018.6372
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发表时间：
2018-06
期刊：
影响因子：
4.2
通讯作者：
Zhang X
中科院分区：
文献类型：
--
作者：
Chen S;Zhang L;Su Y;Zhang X
本研究旨在筛选结直肠癌（CRC）组织及癌旁组织中环状RNA（circRNA）表达的差异，分析相关的miRNAs和mRNAs，探讨circRNA在CRC中的表达及其功能。使用CapitalBio微阵列技术生成circRNA表达谱。用GeneSpring 12.5软件对差异表达的circRNA进行鉴定。随后，使用分子注释系统（MAS）3.0注释差异表达的circRNA的相关mRNA，并使用KEGG基于同源性的注释系统（KOBAS）3.0对这些mRNA进行疾病、通路和功能富集分析。此外，利用米兰达算法筛选差异表达circRNA的靶miRNAs。使用Cytoscape软件3.4.0构建前8个最显著差异表达的circRNA/miRNA网络。共鉴定出10，245个差异表达的circRNA，其中6，264个上调，3，981个下调。相关mRNA在462种KEGG疾病、411种FunDO、669种NHGRI GWAS目录和845种OMIM中富集; 1，334种反应组、281种KEGG途径、117种PANTHER和193种BioCyc;以及11，606种基因本体论（GO）术语。共有133个circRNA/miRNA对参与了circRNA/miRNA网络。hsa_circ_0126897_CBC1可能是大肠癌的潜在生物标志物，细胞周期与大肠癌的发生发展密切相关。
The aim of the present study was to screen colorectal cancer (CRC) tissue and adjacent tissue for differences in circular RNA (circRNA) expression, to analyze the related miRNAs and messenger RNAs (mRNAs), and to investigate the circRNA expression in CRC and its function. The circRNA expression profile was generated using CapitalBio microarray technology. The differentially expressed circRNAs were identified with GeneSpring 12.5 software. Subsequently, the related mRNAs of the differentially expressed circRNAs were annotated with the molecule annotation system (MAS) 3.0, and the diseases, pathways and functional enrichment analysis of these mRNAs were performed using the KEGG orthology-based annotation system (KOBAS) 3.0. In addition, the target miRNAs of differentially expressed circRNAs were screened using the miRanda algorithm. The circRNA/miRNA network was constructed for the top 8 most significant differentially expressed circRNAs with Cytoscape software 3.4.0. A total of 10,245 differentially expressed circRNAs were identified, including 6,264 upregulated ones, and 3,981 downregulated ones. The related mRNAs were enriched in 462 KEGG diseases, 411 FunDO, 669 NHGRI GWAS catalog, and 845 OMIM; and 1,334 Reactomes, 281 KEGG pathways, 117 PANTHER and 193 BioCyc; and 11,606 Gene Ontology (GO) terms. A total of 133 circRNA/miRNA pairs were involved in the circRNA/miRNA network. hsa_circ_0126897_CBC1 may be a potential biomarker for CRC, and the cell cycle was closely associated with the occurrence and development of CRC.
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