Developmental shifts in gene expression in the auditory forebrain during the sensitive period for song learning.
Developmental shifts in gene expression in the auditory forebrain during the sensitive period for song learning.
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DOI：
10.1002/dneu.20719
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发表时间：
2009-06
影响因子：
3
通讯作者：
Clayton, David F.
中科院分区：
文献类型：
--
作者：
London, Sarah E.;Dong, Shu;Replogle, Kirstin;Clayton, David F.
一只雄性斑胸草雀在青少年发育的敏感时期通过记忆导师的歌曲开始学习唱歌。导师歌曲记忆需要听觉前脑内的分子信号。使用微阵列和原位杂交，我们测试了听觉前脑在辅导之前的年龄是否表达了一组不同的基因，与歌曲学习停止后的生活相比。微阵列分析显示，与成年期相比，在孵化后第20天（P20），雄性听觉前脑中数千个基因的表达存在差异。此外，歌曲回放对P20听觉前脑的基因表达基本上没有影响，但改变了成年人数百个基因的表达。大多数在成年人中对歌曲有反应的基因在P20时以组成性高水平表达。使用原位杂交与44个探针的代表性样品，我们证实了这些影响，并发现鸟类在P20和P45在他们的基因表达模式相似。此外，8个探针显示出表达的雄性-雌性差异。我们的结论是，发展中的听觉前脑是在一个非常不同的分子状态，从成人，尽管其相对成熟的毛形态和电生理反应的歌曲刺激。发育中基因表达的变化可能有助于对歌曲学习所需的细胞和分子特性进行微调。
A male zebra finch begins to learn to sing by memorizing a tutor’s song during a sensitive period in juvenile development. Tutor song memorization requires molecular signaling within the auditory forebrain. Using microarray and in situ hybridizations, we tested whether the auditory forebrain at an age just prior to tutoring expresses a different set of genes compared to later in life after song learning has ceased. Microarray analysis revealed differences in expression of thousands of genes in the male auditory forebrain at posthatch day 20 (P20) compared to adulthood. Further, song playbacks had essentially no impact on gene expression in P20 auditory forebrain, but altered expression of hundreds of genes in adults. Most genes that were song-responsive in adults were expressed at constitutively high levels at P20. Using in situ hybridization with a representative sample of 44 probes, we confirmed these effects and found that birds at P20 and P45 were similar in their gene expression patterns. Additionally, 8 of the probes showed male-female differences in expression. We conclude that the developing auditory forebrain is in a very different molecular state from the adult, despite its relatively mature gross morphology and electrophysiological responsiveness to song stimuli. Developmental gene expression changes may contribute to fine-tuning of cellular and molecular properties necessary for song learning.
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影响因子：
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作者：
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