Miniaturized optical neuroimaging in unrestrained animals.
Miniaturized optical neuroimaging in unrestrained animals.
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DOI：
10.1016/j.neuroimage.2015.02.070
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发表时间：
2015-06
期刊：
影响因子：
5.7
通讯作者：
Pathak AP
中科院分区：
文献类型：
--
作者：
Yu H;Senarathna J;Tyler BM;Thakor NV;Pathak AP
光学技术进步、微型电子元件以及不断增长且负担得起的计算能力的可用性的融合开创了功能神经成像的新时代。也就是说，一个时代，在不受限制和未麻醉的啮齿动物的皮层功能的神经成像已经成为现实。传统的光学神经成像需要动物被麻醉和限制。这极大地限制了可以在体内进行的实验种类。现在，人们可以评估功能活动引起的血流和氧合变化，成像中风和癫痫发作等疾病模型的功能反应，甚至进行肿瘤生理学的长期成像，所有这些都没有麻醉剂或动物限制的混淆作用。这些进展为哺乳动物脑组织和功能提供了新的线索，并有助于阐明在广泛的中枢神经系统疾病模型中这种组织的丧失或“功能障碍”。在这篇综述中，我们强调了最近的进展，在制造，表征和应用的小型化头戴式光学神经成像系统开创了创新的研究人员从广泛的学科。我们将这些系统大致分为基于外源性造影剂的系统，如单光子和双光子显微镜系统;以及基于内源性对比机制的系统，如多光谱或激光散斑对比成像系统。最后，我们将讨论这些方法的优点和缺点，沿着展望神经影像学这一令人兴奋的新领域的未来。
The confluence of technological advances in optics, miniaturized electronic components and the availability of ever increasing and affordable computational power have ushered in a new era in functional neuroimaging. Namely, an era in which neuroimaging of cortical function in unrestrained and unanesthetized rodents has become a reality. Traditional optical neuroimaging required animals to be anesthetized and restrained. This greatly limited the kinds of experiments that could be performed in vivo. Now one can assess blood flow and oxygenation changes resulting from functional activity, image functional response in disease models such as stroke and seizure, and even conduct long-term imaging of tumor physiology, all without the confounding effects of anesthetics or animal restraints. These advances are shedding new light on mammalian brain organization and function, and helping to elucidate loss of this organization or ‘dysfunction’ in a wide array of central nervous system disease models. In this review, we highlight recent advances in the fabrication, characterization and application of miniaturized head-mounted optical neuroimaging systems pioneered by innovative investigators from a wide array of disciplines. We broadly classify these systems into those based on exogenous contrast agents, such as single- and two-photon microscopy systems; and those based on endogenous contrast mechanisms, such as multispectral or laser speckle contrast imaging systems. Finally, we conclude with a discussion of the strengths and weaknesses of these approaches along with a perspective on the future of this exciting new frontier in neuroimaging.
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DOI：
10.1364/josaa.25.002088
发表时间：
2008-08
期刊：
Journal of the Optical Society of America. A, Optics, image science, and vision
影响因子：
--
作者：
Duncan DD;Kirkpatrick SJ
通讯作者：
Kirkpatrick SJ
影响因子：
25
作者：
Dombeck, Daniel A.;Harvey, Christopher D.;Tian, Lin;Looger, Loren L.;Tank, David W.
通讯作者：
Tank, David W.
影响因子：
3.5
作者：
Bassi, Andrea;Fieramonti, Luca;Valentini, Gianluca
通讯作者：
Valentini, Gianluca
影响因子：
48
作者：
Barretto, Robert P. J.;Messerschmidt, Bernhard;Schnitzer, Mark J.
通讯作者：
Schnitzer, Mark J.
影响因子：
3.6
作者：
Flusberg, BA;Lung, JC;Schnitzer, MJ
通讯作者：
Schnitzer, MJ