Stability of healthy subgingival microbiome across space and time.
Stability of healthy subgingival microbiome across space and time.
复制标题
DOI：
10.1038/s41598-021-03479-2
复制
发表时间：
2021-12-14
影响因子：
4.6
通讯作者：
Wang GP
中科院分区：
文献类型：
--
作者：
Tamashiro R;Strange L;Schnackenberg K;Santos J;Gadalla H;Zhao L;Li EC;Hill E;Hill B;Sidhu G;Kirst M;Walker C;Wang GP
龈下微生物组是人体内最稳定的微生物生态系统之一。龈下微生物组的改变与牙周疾病有关，但它们随时间的变化以及牙周健康个体不同龈下部位之间的变化尚未得到很好的描述。我们对5名牙周健康个体的龈下微生物组进行了广泛的纵向采样，以确定基线空间和时间变化。在6-12个月内收集了5名受试者的共251份龈下样本，并进行了深度测序。个体之间的总体微生物多样性和组成差异显着。在每个个体中，我们观察到不同龈下部位之间的微生物组组成存在相当大的差异。然而，对于一个给定的位置，微生物组随着时间的推移是非常稳定的，这种稳定性与微生物多样性的增加有关，但与推定的牙周病原体的富集呈负相关。与微生物组组成相反，预测的功能宏基因组在空间和时间上是相似的，这表明牙周健康与由个性化的不同微生物组财团编码的共享基因功能相关。据我们所知，这是迄今为止对健康龈下微生物组最详细的纵向分析之一，该分析检查了个体内不同龈下部位的纵向变异性。这些结果表明，在给定部位的健康龈下微生物组的单次测量可以提供微生物组成和功能潜力的长期信息，但每个部位的采样对于确定各个龈下部位的组成和群落结构是必要的。
The subgingival microbiome is one of the most stable microbial ecosystems in the human body. Alterations in the subgingival microbiome have been associated with periodontal disease, but their variations over time and between different subgingival sites in periodontally healthy individuals have not been well described. We performed extensive, longitudinal sampling of the subgingival microbiome from five periodontally healthy individuals to define baseline spatial and temporal variations. A total of 251 subgingival samples from 5 subjects were collected over 6–12 months and deep sequenced. The overall microbial diversity and composition differed significantly between individuals. Within each individual, we observed considerable differences in microbiome composition between different subgingival sites. However, for a given site, the microbiome was remarkably stable over time, and this stability was associated with increased microbial diversity but was inversely correlated with the enrichment of putative periodontal pathogens. In contrast to microbiome composition, the predicted functional metagenome was similar across space and time, suggesting that periodontal health is associated with shared gene functions encoded by different microbiome consortia that are individualized. To our knowledge, this is one of the most detailed longitudinal analysis of the healthy subgingival microbiome to date that examined the longitudinal variability of different subgingival sites within individuals. These results suggest that a single measurement of the healthy subgingival microbiome at a given site can provide long term information of the microbial composition and functional potential, but sampling of each site is necessary to define the composition and community structure at individual subgingival sites.
登录
查看更多内容
影响因子：
5.4
作者：
Han YW
通讯作者：
Han YW
影响因子：
9.2
作者：
Hall MW;Singh N;Ng KF;Lam DK;Goldberg MB;Tenenbaum HC;Neufeld JD;G Beiko R;Senadheera DB
通讯作者：
Senadheera DB
影响因子：
3.7
作者：
Erb-Downward JR;Thompson DL;Han MK;Freeman CM;McCloskey L;Schmidt LA;Young VB;Toews GB;Curtis JL;Sundaram B;Martinez FJ;Huffnagle GB
通讯作者：
Huffnagle GB
影响因子：
17.1
作者：
Gajer P;Brotman RM;Bai G;Sakamoto J;Schütte UM;Zhong X;Koenig SS;Fu L;Ma ZS;Zhou X;Abdo Z;Forney LJ;Ravel J
通讯作者：
Ravel J
影响因子：
16.6
作者：
Proctor DM;Fukuyama JA;Loomer PM;Armitage GC;Lee SA;Davis NM;Ryder MI;Holmes SP;Relman DA
通讯作者：
Relman DA