Field evaluation of specific mycobacterial protein-based skin test for the differentiation of Mycobacterium bovis-infected and Bacillus Calmette Guerin-vaccinated crossbred cattle in Ethiopia.
Field evaluation of specific mycobacterial protein-based skin test for the differentiation of Mycobacterium bovis-infected and Bacillus Calmette Guerin-vaccinated crossbred cattle in Ethiopia.
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埃塞俄比亚牛分枝杆菌感染和卡介苗接种杂交牛特异性分枝杆菌蛋白皮肤试验的现场评价。
DOI：
10.1111/tbed.14252
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发表时间：
2022-07
影响因子：
4.3
通讯作者：
Ameni G
中科院分区：
文献类型：
--
作者：
Bayissa B;Sirak A;Zewude A;Worku A;Gumi B;Berg S;Hewinson RG;Wood JLN;Jones GJ;ETHICOBOTS consortium;Vordermeier HM;Ameni G
牛结核病（bTB）对埃塞俄比亚的集约化乳制品生产提出了挑战，在该国实施检测和屠宰控制战略在经济上是不可接受的。用卡介苗（BCG）接种牛可能是控制的重要辅助手段，这需要诊断试验来区分牛分枝杆菌（M。牛）感染和BCG接种的动物（DIVA作用）。本研究描述了基于特定（ESAT-6、CFP-10和Rv 3615 c）M的混合物（DSTc）或融合物（DSTf）的DIVA皮肤试验（DST）的评价。牛蛋白质的Zebu-Holstein-Friesians杂交牛在埃塞俄比亚。使用的研究动物为74头实验开始时年龄小于3周龄的小牛（35头接种BCG，39头未接种）和68头自然感染的“TB反应”奶牛。接种后6周，对74头小牛进行DSTc和单次皮内宫颈比较结核菌素（SICCT）试验。使用DSTc和SICCT测试对TB反应奶牛进行测试。在BCG接种和未接种小牛中未观察到对DSTc的反应，而在接种小牛中检测到SICCT试验反应。在95.6%的TB反应牛中检测到DSTc反应，在98.2%的TB反应牛中发现单一皮内结核菌素阳性反应（95%置信区间，CI，92.1-100%）。DSTc的灵敏度为95.6%（95% CI，87.6-99.1%），显著（p <0.001）高于SICCT测试在4 mm临界值时的灵敏度（75%，95% CI，63.0-84.7%）。DSTf和DSTc反应相关（r = 0.75; 95%CI = 0.53-0.88）。结论：DSTc可区分M.在埃塞俄比亚接种卡介苗的牛身上感染的。DST的敏感性高于SICCT。因此，如果在埃塞俄比亚和其他国家实施卡介苗接种以控制bTB，DSTc可用作bTB的诊断工具。
Bovine tuberculosis (bTB) challenges intensive dairy production in Ethiopia and implementation of the test and slaughter control strategy is not economically acceptable in the country. Vaccination of cattle with Bacillus Calmette–Guerin (BCG) could be an important adjunct to control, which would require a diagnostic test to differentiate Mycobacterium bovis (M. bovis)‐infected and BCG‐vaccinated animals (DIVA role). This study describes an evaluation of a DIVA skin test (DST) that is based on a cocktail (DSTc) or fusion (DSTf) of specific (ESAT‐6, CFP‐10 and Rv3615c) M. bovis proteins in Zebu–Holstein–Friesians crossbred cattle in Ethiopia. The study animals used were 74 calves (35 BCG vaccinated and 39 unvaccinated) aged less than 3 weeks at the start of experiment and 68 naturally infected ‘TB reactor’ cows. Six weeks after vaccination, the 74 calves were tested with the DSTc and the single intradermal cervical comparative tuberculin (SICCT) test. The TB reactor cows were tested with the DSTc and the SICCT test. Reactions to the DSTc were not observed in BCG‐vaccinated and unvaccinated calves, while SICCT test reactions were detected in vaccinated calves. DSTc reactions were detected in 95.6% of the TB reactor cows and single intradermal tuberculin positive reactions were found in 98.2% (95% confidence interval, CI, 92.1–100%). The sensitivity of the DSTc was 95.6% (95% CI, 87.6–99.1%), and significantly (p < .001) higher than the sensitivity (75%, 95% CI, 63.0–84.7%) of the SICCT test at 4 mm cut‐off. DSTf and DSTc reactions were correlated (r = 0.75; 95% CI = 0.53–0.88). In conclusion, the DSTc could differentiate M. bovis‐infected from BCG‐vaccinated cattle in Ethiopia. DST had higher sensitivity than the SICCT test. Hence, the DSTc could be used as a diagnostic tool for bTB if BCG vaccination is implemented for the control of bTB in Ethiopia and other countries.
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影响因子：
2.4
作者：
Bezos, Javier;Casal, Carmen;Dominguez, Lucas
通讯作者：
Dominguez, Lucas
DOI：
10.1128/cdli.8.3.571-578.2001
发表时间：
2001-05-01
期刊：
CLINICAL AND DIAGNOSTIC LABORATORY IMMUNOLOGY
影响因子：
--
作者：
Vordermeier, HM;Whelan, A;Hewinson, RG
通讯作者：
Hewinson, RG
DOI：
10.1128/cdli.6.5.675-682.1999
发表时间：
1999-09-01
期刊：
CLINICAL AND DIAGNOSTIC LABORATORY IMMUNOLOGY
影响因子：
--
作者：
Vordermeier, HM;Cockle, PC;Hewinson, RG
通讯作者：
Hewinson, RG
影响因子：
3.1
作者：
Sidders, Ben;Pirson, Chris;Ewer, Katie
通讯作者：
Ewer, Katie
影响因子：
4.3
作者：
Ameni G;Tafess K;Zewde A;Eguale T;Tilahun M;Hailu T;Sirak A;Salguero FJ;Berg S;Aseffa A;Hewinson RG;Vordermeier HM
通讯作者：
Vordermeier HM