Basophil reactivity, wheal size, and immunoglobulin levels distinguish degrees of cow's milk tolerance.
Basophil reactivity, wheal size, and immunoglobulin levels distinguish degrees of cow's milk tolerance.
复制标题
DOI：
10.1016/j.jaci.2012.06.003
复制
发表时间：
2013-01
影响因子：
14.2
通讯作者：
Sampson, Hugh A.
中科院分区：
文献类型：
--
作者：
Ford, Lara S.;Bloom, Katherine A.;Nowak-Wegrzyn, Anna H.;Shreffler, Wayne G.;Masilamani, Madhan;Sampson, Hugh A.
关键词：
在我们之前的研究中，大约75%的牛奶过敏儿童耐受烘焙奶制品，这改善了他们的预后和生活质量。我们试图在一组牛奶过敏儿童中确定不同程度的临床耐受性的生物标志物。132名受试者最初根据口服食物挑战被分类为烘焙牛奶反应性、烘焙牛奶耐受性或“过度牛奶过敏”。然后，基于它们能够耐受的热变性乳蛋白的量和程度，将烘焙乳耐受组分成3组。分析血清中的过敏原特异性IgE和IgG 4，在用连续10倍稀释的牛奶蛋白刺激的全血中评估嗜碱性粒细胞反应性，并对商业牛奶提取物进行点刺皮肤试验。使用流式细胞术将活化的嗜碱性粒细胞定义为CD 63 brightCD 203 c + CD 123 +HLA-DRdim/− CD 41 a − lineage−。使用Jonckheere-Terpstra检验分析数据。在5个临床组中观察到酪蛋白和乳汁特异性IgE、酪蛋白特异性IgG 4和酪蛋白IgE/IgG 4的中位值、乳汁特异性与非特异性嗜碱性粒细胞活化比率、嗜碱性粒细胞反应性中位值和自发嗜碱性粒细胞活化（RPMI刺激后的CD 203 c表达）以及乳汁PST风团直径存在显著差异。酪蛋白和牛奶特异性IgE、牛奶特异性嗜碱性粒细胞反应性和牛奶点刺皮肤试验风团直径在对烘焙牛奶有反应的牛奶过敏患者中均显著大于对烘焙牛奶有反应的牛奶过敏患者。牛奶过敏儿童的不同表型可以通过酪蛋白和牛奶特异性IgE、牛奶特异性嗜碱性粒细胞反应性和牛奶点刺皮肤试验平均风团直径来区分。自发嗜碱性粒细胞活化是更严重的临床牛奶反应的患者。
In our previous study, about 75% of cow’s milk-allergic children tolerated baked-milk products, which improved their prognosis and quality of life. We sought to identify biomarkers of varying degrees of clinical tolerance among a cohort of cow’s milk-allergic children. 132 subjects were initially classified as baked-milk-reactive, baked-milk-tolerant or “outgrown milk allergy” based on oral food challenges. The baked-milk tolerant group was then divided into 3 groups based upon the amount and degree of heat-denatured milk protein that they could tolerate. Serum was analyzed for allergen-specific IgE and IgG4, basophil reactivity was assessed in whole blood stimulated with serial 10-fold dilutions of milk protein, and prick skin tests were performed to commercial milk extract. Activated basophils were defined using flow cytometry as CD63brightCD203c+CD123+HLA-DRdim/−CD41a− lineage−. Data were analyzed using the Jonckheere-Terpstra test. Significant differences across the five clinical groups were seen for median casein- and milk-specific IgE, casein-specific IgG4 and casein IgE/IgG4; milk-specific to non-specific basophil activation ratio, median basophil reactivity, and spontaneous basophil activation (CD203c expression following stimulation with RPMI); and milk PST wheal diameters. Casein- and milk-specific IgE, milk-specific basophil reactivity and milk prick skin test wheal diameter are all significantly greater among milk-allergic patients who react to baked-milk than among those who tolerate it. The majority of milk-allergic patients are able to tolerate some forms of baked-milk in their diets. Different phenotypes of cow’s milk-allergic children can be distinguished by casein- and milk-specific IgE, milk-specific basophil reactivity, and milk prick skin test mean wheal diameters. Spontaneous basophil activation is greater among patients with more severe clinical milk reactivity.
登录
查看更多内容
DOI：
10.1016/j.jaci.2010.10.007
发表时间：
2010-12
期刊：
The Journal of allergy and clinical immunology
影响因子：
--
作者：
NIAID-Sponsored Expert Panel;Boyce JA;Assa'ad A;Burks AW;Jones SM;Sampson HA;Wood RA;Plaut M;Cooper SF;Fenton MJ;Arshad SH;Bahna SL;Beck LA;Byrd-Bredbenner C;Camargo CA Jr;Eichenfield L;Furuta GT;Hanifin JM;Jones C;Kraft M;Levy BD;Lieberman P;Luccioli S;McCall KM;Schneider LC;Simon RA;Simons FE;Teach SJ;Yawn BP;Schwaninger JM
通讯作者：
Schwaninger JM
影响因子：
14.2
作者：
Skripak, Justin M.;Matsui, Elizabeth C.;Wood, Robert A.
通讯作者：
Wood, Robert A.
DOI：
10.1111/j.1365-2222.1977.tb01466.x
发表时间：
1977-01-01
期刊：
CLINICAL ALLERGY
影响因子：
--
作者：
BOCK, SA;BUCKLEY, J;MAY, CD
通讯作者：
MAY, CD
DOI：
10.1159/000233761
发表时间：
1985-01-01
期刊：
INTERNATIONAL ARCHIVES OF ALLERGY AND APPLIED IMMUNOLOGY
影响因子：
--
作者：
MARONE, G;POTO, S;BONINI, S
通讯作者：
BONINI, S
影响因子：
14.2
作者：
Shreffler, Wayne G.;Wanich, Niya;Sampson, Hugh A.
通讯作者：
Sampson, Hugh A.