Characterization of Pax3 and Sox10 transgenic Xenopus laevis embryos as tools to study neural crest development.
Characterization of Pax3 and Sox10 transgenic Xenopus laevis embryos as tools to study neural crest development.
复制标题
DOI：
10.1016/j.ydbio.2018.02.020
复制
发表时间：
2018-12-01
影响因子：
2.7
通讯作者：
Mayor R
中科院分区：
文献类型：
--
作者：
Alkobtawi M;Ray H;Barriga EH;Moreno M;Kerney R;Monsoro-Burq AH;Saint-Jeannet JP;Mayor R
神经脊是起源于各种细胞类型的多潜能细胞群体。许多动物模型被用来研究神经脊的诱导、迁移和分化，其中两栖动物和鸟类是最广泛使用的系统。研究小鼠、小鸡和斑马鱼神经脊发育的一个主要技术进步是产生了转基因动物，在转基因动物中，神经脊特异性增强子/启动子驱动荧光蛋白的表达作为谱系示踪剂，或者用于功能研究的修饰基因的表达。不幸的是，非洲爪哇和热带两栖动物目前还没有这样的转基因动物，这两种动物是研究神经脊发育的关键模型系统。在这里，我们描述了两个转基因非洲爪哇品系Pax3-GFP和Sox10-GFP的产生和特性，其中GFP分别在迁移前和迁移的神经脊中表达。结果表明，Pax3-GFP可作为研究神经峰诱导的有力工具，而Sox10-GFP可用于活体胚胎神经峰迁移的研究。Pax3-GFP非洲爪哇转基因表达GFP在迁移前神经脊中表达Pax3-GFP非洲爪哇对Wnt信号的响应Sox10-GFP非洲爪哇转基因表达GFP迁移神经脊Pax3-GFP和Sox10-GFP非洲爪哇是研究神经脊诱导和迁移的潜在工具
The neural crest is a multipotent population of cells that originates a variety of cell types. Many animal models are used to study neural crest induction, migration and differentiation, with amphibians and birds being the most widely used systems. A major technological advance to study neural crest development in mouse, chick and zebrafish has been the generation of transgenic animals in which neural crest specific enhancers/promoters drive the expression of either fluorescent proteins for use as lineage tracers, or modified genes for use in functional studies. Unfortunately, no such transgenic animals currently exist for the amphibians Xenopus laevis and tropicalis, key model systems for studying neural crest development. Here we describe the generation and characterization of two transgenic Xenopus laevis lines, Pax3-GFP and Sox10-GFP, in which GFP is expressed in the pre-migratory and migratory neural crest, respectively. We show that Pax3-GFP could be a powerful tool to study neural crest induction, whereas Sox10-GFP could be used in the study of neural crest migration in living embryos. Pax3-GFP Xenopus laves transgenic expresses GFP in pre-migratory neural crest Pax3-GFP Xenopus laevis transgenic responds to Wnt signalling Sox10-GFP Xenopus laevis transgenic expresses GFP in migrating neural crest Pax3-GFP and Sox10-GFP Xenopus laevis transgenic represent potential tools to study neural crest induction and migration
登录
查看更多内容
影响因子：
4.6
作者：
Mayor, Roberto;Theveneau, Eric
通讯作者：
Theveneau, Eric
影响因子：
11.8
作者：
Sauka-Spengler, Tatjana;Meulemans, Daniel;Bronner-Fraser, Marianne
通讯作者：
Bronner-Fraser, Marianne
影响因子：
1.5
作者：
Rodrigues, Frederico S. L. M.;Doughton, Gail;Kelsh, Robert N.
通讯作者：
Kelsh, Robert N.
DOI：
10.1126/science.aaf2729
发表时间：
2016-06-24
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Simoes-Costa M;Bronner ME
通讯作者：
Bronner ME
影响因子：
3.6
作者：
Shibata S;Yasuda A;Renault-Mihara F;Suyama S;Katoh H;Inoue T;Inoue YU;Nagoshi N;Sato M;Nakamura M;Akazawa C;Okano H
通讯作者：
Okano H