Temporal analysis of ectopic enamel production in incisors from sprouty mutant mice.
Temporal analysis of ectopic enamel production in incisors from sprouty mutant mice.
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DOI：
10.1002/jez.b.21254
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发表时间：
2009-07-15
影响因子：
2.2
通讯作者：
Klein, Ophir D.
中科院分区：
文献类型：
--
作者：
Boran, Tomas;Peterkova, Renata;Lesot, Herve;Lyons, David B.;Peterka, Miroslav;Klein, Ophir D.
老鼠的门牙有两个不寻常的特点：它不断增长，它是由搪瓷专门覆盖在唇侧。持续生长部分是由宫颈环区域的上皮干细胞驱动的，这些上皮干细胞可以自我更新并产生成釉细胞。我们以前曾报道过，异位釉质被发现在与发芽（spry）基因功能丧失的小鼠的门牙的舌侧。Spry2 +/−; Spry4 −/−小鼠，其中三个sprougty等位基因已经失活，由于宫颈环舌部上皮-间充质FGF信号转导的上调而具有异位釉质。有趣的是，Spry4 −/−小鼠的舌釉质也存在于出生后早期，其中只有两个发芽等位基因被灭活，但在这种基因型的成年人中没有发现异位釉质。为了探索Spry4 −/−成年人舌釉质消失的潜在机制，我们通过在围产期和成年期之间的几个时间点比较Spry4 −/−小鼠与野生型和Spry2 +/−; Spry4 −/−小鼠下切牙的形态和生长，研究了Spry4 −/−小鼠舌釉质的命运。在出生后Spry2 +/−; Spry4 −/+切牙的舌侧检测到成釉细胞和釉质。相比之下，Spry4 −/−切牙出生后第3天，新的异位成釉细胞停止分化，随后舌釉逐渐丧失。在Spry4 −/−切牙中，舌侧釉质的后部范围及其最后沉积的时间在出生后早期是可变的，但在所有Spry4 −/−成人切牙中，舌侧釉质最终通过切牙的持续生长和磨损而丢失。
The mouse incisor has two unusual features: it grows continuously and it is covered by enamel exclusively on the labial side. The continuous growth is driven in part by epithelial stem cells in the cervical loop region that can both self-renew and give rise to ameloblasts. We have previously reported that ectopic enamel is found on the lingual side of the incisor in mice with loss-of-function of sprouty (spry) genes. Spry2+/−; Spry4−/− mice, in which three sprouty alleles have been inactivated, have ectopic enamel as a result of upregulation of epithelial-mesenchymal FGF signaling in the lingual part of the cervical loop. Interestingly, lingual enamel is also present in the early postnatal period in Spry4−/− mice, in which only two sprouty alleles have been inactivated, but ectopic enamel is not found in adults of this genotype. To explore the mechanisms underlying the disappearance of lingual enamel in Spry4−/− adults, we studied the fate of the lingual enamel in Spry4−/− mice by comparing the morphology and growth of their lower incisors with wild type and Spry2+/−; Spry4−/− mice at several timepoints between the perinatal period and adulthood. Ameloblasts and enamel were detected on the lingual side in postnatal Spry2+/−; Spry4−/+ incisors. By contrast, new ectopic ameloblasts ceased to differentiate after postnatal day 3 in Spry4−/− incisors, which was followed by a progressive loss of lingual enamel. Both the posterior extent of lingual enamel and the time of its last deposition were variable early postnatally in Spry4−/− incisors, but in all Spry4−/− adult incisors the lingual enamel was ultimately lost through continuous growth and abrasion of the incisor.
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影响因子：
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通讯作者：
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作者：
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