Cortical Up states induce the selective weakening of subthreshold synaptic inputs.
Cortical Up states induce the selective weakening of subthreshold synaptic inputs.
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DOI：
10.1038/s41467-017-00748-5
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发表时间：
2017-09-22
影响因子：
16.6
通讯作者：
Mann EO
中科院分区：
文献类型：
--
作者：
Bartram J;Kahn MC;Tuohy S;Paulsen O;Wilson T;Mann EO
慢波睡眠被认为对皮层突触的重新调整很重要，但其细胞机制仍然没有解决。在慢波活动期间，皮层神经元显示去极化向上状态和超极化向下状态之间的同步转换。在这里，使用记录从LIII锥体神经元从急性切片的小鼠内侧内嗅皮层，我们发现，阈下输入到达期间的状态进行突触减弱。这并不反映全局突触降尺度的过程，因为它依赖于突触前尖峰，网络状态编码在突触诱发的脊髓Ca 2+响应中。我们的数据表明，突触弱化的诱导是在突触后控制下，因为它可以通过相关的突触后尖峰活动来阻止，并且依赖于突触后NMDA受体和GSK 3 β活性。这提供了一种机制，通过这种机制，慢波活动可能会使突触偏向减弱，同时保留活跃神经元组装内的突触连接。皮质上和下状态之间的缓慢振荡是深度睡眠的一个定义特征，但它们的功能还不清楚。在这里，作者研究了内嗅皮层急性切片的Up/Down状态，发现Up状态促进阈下突触输入的减弱，而阈上输入被保留或加强。
Slow-wave sleep is thought to be important for retuning cortical synapses, but the cellular mechanisms remain unresolved. During slow-wave activity, cortical neurons display synchronized transitions between depolarized Up states and hyperpolarized Down states. Here, using recordings from LIII pyramidal neurons from acute slices of mouse medial entorhinal cortex, we find that subthreshold inputs arriving during the Up state undergo synaptic weakening. This does not reflect a process of global synaptic downscaling, as it is dependent on presynaptic spiking, with network state encoded in the synaptically evoked spine Ca2+ responses. Our data indicate that the induction of synaptic weakening is under postsynaptic control, as it can be prevented by correlated postsynaptic spiking activity, and depends on postsynaptic NMDA receptors and GSK3β activity. This provides a mechanism by which slow-wave activity might bias synapses towards weakening, while preserving the synaptic connections within active neuronal assemblies. Slow oscillations between cortical Up and Down states are a defining feature of deep sleep, but their function is not well understood. Here the authors study Up/Down states in acute slices of entorhinal cortex, and find that Up states promote the weakening of subthreshold synaptic inputs, while suprathreshold inputs are preserved or strengthened.
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影响因子：
3.7
作者：
Clopath C;Gerstner W
通讯作者：
Gerstner W
影响因子：
3.1
作者：
Canto CB;Wouterlood FG;Witter MP
通讯作者：
Witter MP
影响因子：
4.8
作者：
Bradley CA;Peineau S;Taghibiglou C;Nicolas CS;Whitcomb DJ;Bortolotto ZA;Kaang BK;Cho K;Wang YT;Collingridge GL
通讯作者：
Collingridge GL
影响因子：
5.6
作者：
de Vivo, Luisa;Faraguna, Ugo;Cirelli, Chiara
通讯作者：
Cirelli, Chiara
影响因子：
16.2
作者：
Isomura, Yoshikazu;Sirota, Anton;Buzsaki, Gyoergy
通讯作者：
Buzsaki, Gyoergy