A combination of Notch signaling, preferential adhesion and endocytosis induces a slow mode of cell intercalation in the Drosophila retina.
A combination of Notch signaling, preferential adhesion and endocytosis induces a slow mode of cell intercalation in the Drosophila retina.
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Notch信号，优先粘附和内吞作用的结合诱导果蝇视网膜的细胞插入模式缓慢。
DOI：
10.1242/dev.197301
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发表时间：
2021-05-15
期刊：
影响因子：
--
通讯作者：
Pichaud F
中科院分区：
文献类型：
--
作者：
Blackie L;Tozluoglu M;Trylinski M;Walther RF;Schweisguth F;Mao Y;Pichaud F
组织中的上皮细胞通过邻居交换发生运动，并驱动组织形状的变化。它需要细胞间连接重塑，这一过程通常由收缩的肌球蛋白细胞骨架提供动力。这主要是在均质上皮细胞中进行的研究，在这种情况下插入需要几分钟的时间。然而，在一些组织中，嵌入涉及不同类型的细胞，可能需要数小时。慢速插层和快速插层是否具有相同的机制仍有待研究。为了解决这个问题，我们使用了苍蝇眼，其中锥体细胞通过∼10 h交换邻居来塑造晶状体。我们发现了三条调节这种缓慢的细胞嵌入模式的途径。首先，我们发现对MyosinII的需求有限。在这种情况下，数学模型预测了一种以粘着为主的插层机制。基因实验支持了这一预测，揭示了通过Nephin蛋白Rroughest和Hibris进行黏附的作用。第二，我们发现锥体细胞的嵌入受Notch通路的调控。第三，我们证明内吞作用是膜去除和Notch激活所必需的。综上所述，我们的工作表明，在组织形态发生过程中，黏附、内吞和Notch可以指导缓慢的细胞嵌入。摘要：通过细胞间连接重塑的细胞嵌入可在几分钟甚至几小时内发生。在果蝇视网膜的视锥细胞中，有三种途径，即黏附、内吞和Notch，可以诱导缓慢嵌入。
Movement of epithelial cells in a tissue occurs through neighbor exchange and drives tissue shape changes. It requires intercellular junction remodeling, a process typically powered by the contractile actomyosin cytoskeleton. This has been investigated mainly in homogeneous epithelia, where intercalation takes minutes. However, in some tissues, intercalation involves different cell types and can take hours. Whether slow and fast intercalation share the same mechanisms remains to be examined. To address this issue, we used the fly eye, where the cone cells exchange neighbors over ∼10 h to shape the lens. We uncovered three pathways regulating this slow mode of cell intercalation. First, we found a limited requirement for MyosinII. In this case, mathematical modeling predicts an adhesion-dominant intercalation mechanism. Genetic experiments support this prediction, revealing a role for adhesion through the Nephrin proteins Roughest and Hibris. Second, we found that cone cell intercalation is regulated by the Notch pathway. Third, we show that endocytosis is required for membrane removal and Notch activation. Taken together, our work indicates that adhesion, endocytosis and Notch can direct slow cell intercalation during tissue morphogenesis. Summary: Cell intercalation via intercellular junction remodeling can occur over minutes or even hours. Three pathways, adhesion, endocytosis and Notch, were found to induce slow intercalation in cone cells of the fly retina.
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