Migration of connective tissue-derived cells is mediated by ultra-low concentration gradient fields of EGF.
Migration of connective tissue-derived cells is mediated by ultra-low concentration gradient fields of EGF.
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DOI：
10.1016/j.yexcr.2011.04.003
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发表时间：
2011-07-01
影响因子：
3.7
通讯作者：
Vazquez M
中科院分区：
文献类型：
--
作者：
Kong Q;Majeska RJ;Vazquez M
细胞向化学刺激的定向迁移包括趋化剂浓度及其浓度梯度的同时变化，其中每一个都可能影响细胞迁移反应。在这项研究中，我们利用微流控系统来研究表皮生长因子（EGF）浓度和EGF梯度之间的相互作用，刺激结缔组织来源的成纤维细胞的趋化性。将接种在微流体装置内的细胞暴露于由EGF浓度建立的浓度梯度，所述浓度梯度匹配或超过在transfilter迁移测定中观察到的最大趋化反应所需的浓度梯度。在实验建立稳态梯度后，光学测量装置内单个细胞的迁移。结果表明，对于所有使用的浓度，在0.01-和0.1-ng/（mL.mm）之间的EGF浓度梯度下，运动性最大。与此相反，运动细胞的数量不断增加，增加梯度陡度的所有浓度检查。基于微流体的实验将细胞暴露于EGF浓度和梯度的微小变化，这些变化与所测量的急性EGFR磷酸化一致。实验数据与建立的数学模型的相关性表明，研究的成纤维细胞表现出未报道的化学敏感性，EGF浓度的微小变化，类似于高度能动的细胞，如巨噬细胞。我们的研究结果表明，浅的趋化梯度，而以前未探索的，是必要的诱导率的定向细胞迁移和运动细胞的数量在结缔组织来源的细胞检查。
The directed migration of cells towards chemical stimuli incorporates simultaneous changes in both the concentration of a chemotactic agent and its concentration gradient, each of which may influence cell migratory response. In this study, we utilized a microfluidic system to examine the interactions between Epidermal Growth Factor (EGF) concentration and EGF gradient in stimulating the chemotaxis of connective-tissue derived fibroblast cells. Cells seeded within microfluidic devices were exposed to concentration gradients established by EGF concentrations that matched or exceeded those required for maximum chemotactic responses seen in transfilter migration assays. The migration of individual cells within the device was measured optically after steady-state gradients had been experimentally established. Results illustrate that motility was maximal at EGF concentration gradients between .01- and 0.1-ng/(mL.mm) for all concentrations used. In contrast, the numbers of motile cells continually increased with increasing gradient steepness for all concentrations examined. Microfluidics-based experiments exposed cells to minute changes in EGF concentration and gradient that were in line with the acute EGFR phosphorylation measured. Correlation of experimental data with established mathematical models illustrated that the fibroblasts studied exhibit an unreported chemosensitivity to minute changes in EGF concentration, similar to that reported for highly motile cells, such as macrophages. Our results demonstrate that shallow chemotactic gradients, while previously unexplored, are necessary to induce the rate of directed cellular migration and the number of motile cells in the connective tissue-derived cells examined.
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DOI：
10.1083/jcb.200204153
发表时间：
2002-11-25
期刊：
The Journal of cell biology
影响因子：
--
作者：
Galbraith CG;Yamada KM;Sheetz MP
通讯作者：
Sheetz MP
影响因子：
4
作者：
Paguirigan, Amy L.;Beebe, David J.
通讯作者：
Beebe, David J.
影响因子：
--
作者：
Kleinman, H K;Jacob, K
通讯作者：
Jacob, K
影响因子：
3.4
作者：
Dalous, Jeremie;Burghardt, Emmanuel;Bretschneider, Till
通讯作者：
Bretschneider, Till
DOI：
10.1164/ajrccm/139.2.320
发表时间：
1989-02-01
期刊：
AMERICAN REVIEW OF RESPIRATORY DISEASE
影响因子：
--
作者：
GLASGOW, JE;FARRELL, BE;DANIELE, RP
通讯作者：
DANIELE, RP