Application of detergents or high hydrostatic pressure as decellularization processes in uterine tissues and their subsequent effects on in vivo uterine regeneration in murine models.
Application of detergents or high hydrostatic pressure as decellularization processes in uterine tissues and their subsequent effects on in vivo uterine regeneration in murine models.
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DOI：
10.1371/journal.pone.0103201
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Furukawa KS
中科院分区：
文献类型：
--
作者：
Santoso EG;Yoshida K;Hirota Y;Aizawa M;Yoshino O;Kishida A;Osuga Y;Saito S;Ushida T;Furukawa KS
卵巢或输卵管问题引起的不孕症可以使用体外受精和胚胎移植（IVF-ET）治疗；然而，对于子宫缺失和畸形的妇女来说，这是不可能的，她们需要重建子宫来治疗不孕症。在这项研究中，我们首次报道了脱细胞基质作为子宫重建支架的有效性。选取SD大鼠子宫组织，在最佳条件下分别用十二烷基硫酸钠（SDS）或高压水静压（HHP）对其进行脱细胞。组织学染色和定量分析表明，SDS和HHP方法都能有效地从组织中去除细胞，特别是HHP构建体的DNA含量显著降低。与SDS构建体相比，HHP构建体高度保留了子宫组织中细胞外基质的主要成分胶原含量，胶原含量与天然组织相似。HHP构建体的机械强度与天然组织相似，SDS构建体的机械强度显著提高。透射电子显微镜（TEM）显示，与SDS结构相比，HHP结构中胶原纤维没有明显变性。将脱细胞组织移植到大鼠子宫，发现移植30天后子宫组织再生成功，具有3层结构。此外，检测到大量上皮腺组织和Ki67阳性细胞。免疫组化分析显示再生组织对妊娠卵巢激素反应正常。移植30天后的妊娠试验显示SDS组和HHP组均成功妊娠。这些结果表明，子宫组织脱细胞基质可作为子宫再生的潜在支架。
Infertility caused by ovarian or tubal problems can be treated using In Vitro Fertilization and Embryo Transfer (IVF-ET); however, this is not possible for women with uterine loss and malformations that require uterine reconstruction for the treatment of their infertility. In this study, we are the first to report the usefulness of decellularized matrices as a scaffold for uterine reconstruction. Uterine tissues were extracted from Sprague Dawley (SD) rats and decellularized using either sodium dodecyl sulfate (SDS) or high hydrostatic pressure (HHP) at optimized conditions. Histological staining and quantitative analysis showed that both SDS and HHP methods effectively removed cells from the tissues with, specifically, a significant reduction of DNA contents for HHP constructs. HHP constructs highly retained the collagen content, the main component of extracellular matrices in uterine tissue, compared to SDS constructs and had similar content levels of collagen to the native tissue. The mechanical strength of the HHP constructs was similar to that of the native tissue, while that of the SDS constructs was significantly elevated. Transmission electron microscopy (TEM) revealed no apparent denaturation of collagen fibers in the HHP constructs compared to the SDS constructs. Transplantation of the decellularized tissues into rat uteri revealed the successful regeneration of the uterine tissues with a 3-layer structure 30 days after the transplantation. Moreover, a lot of epithelial gland tissue and Ki67 positive cells were detected. Immunohistochemical analyses showed that the regenerated tissues have a normal response to ovarian hormone for pregnancy. The subsequent pregnancy test after 30 days transplantation revealed successful pregnancy for both the SDS and HHP groups. These findings indicate that the decellularized matrix from the uterine tissue can be a potential scaffold for uterine regeneration.
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影响因子：
14
作者：
Li, Xinan;Sun, Haixiang;Hu, Yali
通讯作者：
Hu, Yali
影响因子：
--
作者：
Patil M
通讯作者：
Patil M
影响因子：
14
作者：
Sullivan, David C.;Mirmalek-Sani, Sayed-Hadi;Yoo, James J.
通讯作者：
Yoo, James J.
影响因子：
14
作者：
Funamoto, Seiichi;Nam, Kwangwoo;Kishida, Akio
通讯作者：
Kishida, Akio
影响因子：
2.4
作者：
JONKMAN, MF;KAUER, FM;MOLENAAR, I
通讯作者：
MOLENAAR, I