Comparative Analysis of DNA Methylation Reveals Specific Regulations on Ethylene Pathway in Tomato Fruit.
Comparative Analysis of DNA Methylation Reveals Specific Regulations on Ethylene Pathway in Tomato Fruit.
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DNA 甲基化的比较分析揭示了番茄果实中乙烯途径的具体调控
作者：
Zuo J;Wang Y;Zhu B;Luo Y;Wang Q;Gao L
DNA甲基化是表观遗传调控的一个重要特征，在植物CG、CHG和CHH位点的多种生理生化过程中发挥重要作用。LeERF 1是番茄中发现的一种乙烯反应因子，在乙烯信号转导中起重要作用。为探讨乙烯代谢途径中DNA甲基化的特征，以转LeERF 1基因番茄果实为材料，对其基因组序列进行了深度测序和生物信息学分析。甲基化类型以CG最多（71.60-72.80%），CHH最少（10.70-12.50%）。番茄基因组不同区域的甲基化水平不同。对差异甲基化区域（DMR）和差异表达基因（DEG）进行联合分析，共发现3030个差异表达基因，其中包括ACS、ACO、MADS-Box、ERFs和F-box等与乙烯合成和信号转导有关的基因。此外，还揭示了DNA甲基化与microRNAs（miRNAs）之间的关系，为进一步研究乙烯途径中的DNA甲基化和小分子RNAs提供了基础信息。
DNA methylation is an essential feature of epigenetic regulation and plays a role in various physiological and biochemical processes at CG, CHG, and CHH sites in plants. LeERF1 is an ethylene response factor (ERF) found in tomatoes which plays an important role in ethylene signal transduction. To explore the characteristics of DNA methylation in the ethylene pathway, sense-/antisense-LeERF1 transgenic tomato fruit were chosen for deep sequencing and bioinformatics parsing. The methylation type with the greatest distribution was CG, (71.60–72.80%) and CHH was found least frequently (10.70–12.50%). The level of DNA methylation was different among different tomato genomic regions. The differentially methylated regions (DMRs) and the differentially expressed genes (DEGs) were conjointly analyzed and 3030 different expressed genes were found, of which several are involved in ethylene synthesis and signaling transduction (such as ACS, ACO, MADS-Box, ERFs, and F-box). Furthermore, the relationships between DNA methylation and microRNAs (miRNAs) were also deciphered, providing basic information for the further study of DNA methylation and small RNAs involved in the ethylene pathway.
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DOI：
10.1038/nrm4043
发表时间：
2015-09
期刊：
Nature reviews. Molecular cell biology
影响因子：
--
作者：
Du J;Johnson LM;Jacobsen SE;Patel DJ
通讯作者：
Patel DJ
植物铬甲基转移酶的DNA甲基化特征。
影响因子：
4.5
作者：
Gouil Q;Baulcombe DC
通讯作者：
Baulcombe DC
影响因子：
9.4
作者：
Liu, Mingchun;Diretto, Gianfranco;Bouzayen, Mondher
通讯作者：
Bouzayen, Mondher
LeERF1正向调节番茄黄化幼苗、植物发育以及果实成熟和软化的乙烯三重反应
影响因子：
6.2
作者：
Li, Yingcong;Zhu, Benzhong;Luo, Yunbo
通讯作者：
Luo, Yunbo
差异转录组分析揭示了沙棘木蛾和沙棘耐冷性相关的基因
影响因子：
3.7
作者：
Cui M;Hu P;Wang T;Tao J;Zong S
通讯作者：
Zong S