Detection and genotyping of Korean porcine rotaviruses.
Detection and genotyping of Korean porcine rotaviruses.
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DOI：
10.1016/j.vetmic.2010.01.019
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发表时间：
2010-08-26
影响因子：
3.3
通讯作者：
Park SJ
中科院分区：
文献类型：
--
作者：
Kim HJ;Park SI;Ha TP;Jeong YJ;Kim HH;Kwon HJ;Kang MI;Cho KO;Park SJ
猪 A 组轮状病毒 (GARV) 被认为是一种重要的动物病原体，因为它们对养猪业的经济影响及其在人类中引起异源感染的潜力。这项研究检查了来自韩国 6 个省份 143 个农场的 475 个粪便样本。 RT-PCR 和巢式 PCR 使用 GARV VP6 基因特异的引物对在 182 份（38.3%）腹泻粪便样本中检测到 GARV 阳性反应。对从 GARV 阳性粪便中分离出的总共 98 株猪 GARV 菌株进行了 G 和 P 基因分型分析。根据序列和系统发育分析，G 和 P 基因型最主要的组合是 G5P[7]，在 63 个 GARV 毒株中发现（64.3%）。 G和P基因型的其他组合是G8P[7]（16株[16.3%]）、G9P[7]（7株[7.1%]）、G9P[23]（2株[2.0%]）和G8P[1]（1株[1.0%]）。在 3 个 G5、5 个 P[7] 和 1 个 P[1] 菌株中未确定 G 或 P 基因型的对应物。有趣的是，系统发育分析表明，所有韩国 G9 毒株与 VI 系猪和人类病毒的关系比与 GARV 的其他谱系（I-V）和韩国人类 G9 毒株（III 系）的关系更密切。这些结果表明，猪 GARV 感染在韩国腹泻仔猪中很常见。感染菌株具有遗传多样性，包括同源菌株 (G5P[7])、异源菌株 (G8P[1]) 和重配菌株 (G8P[7])，以及新出现的 G9 GARV 菌株。
Porcine group A rotavirus (GARV) is considered to be an important animal pathogen due to their economic impact in the swine industry and its potential to cause heterologous infections in humans. This study examined 475 fecal samples from 143 farms located in 6 provinces across South Korea. RT-PCR and nested PCR utilizing primer pairs specific for the GARV VP6 gene detected GARV-positive reactions in 182 (38.3%) diarrheic fecal samples. A total of 98 porcine GARV strains isolated from the GARV-positive feces were analyzed for G and P genotyping. Based on the sequence and phylogenetic analyses, the most predominant combination of G and P genotypes was G5P[7], found in 63 GARV strains (64.3%). The other combinations of G and P genotypes were G8P[7] (16 strains [16.3%]), G9P[7] (7 strains [7.1%]), G9P[23] (2 strains [2.0%]), and G8P[1] (1 strain [1.0%]). The counterparts of G or P genotypes were not determined in three G5, five P[7], and one P[1] strains. Interestingly, phylogenetic analysis indicated that all Korean G9 strains were more closely related to lineage VI porcine and human viruses than to other lineages (I–V) of GARVs and to Korean human G9 strains (lineage III). These results show that porcine GARV infections are common in diarrheic piglets in South Korea. The infecting strains are genetically diverse, and include homologous (G5P[7]), heterologous (G8P[1]), and reassortant (G8P[7]), as well as emerging G9 GARV strains.
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影响因子：
5
作者：
Fukai, K;Saito, T;Sato, M
通讯作者：
Sato, M
影响因子：
3.3
作者：
Alferi, AF;Alfieri, AA;Richtzenhain, LJ
通讯作者：
Richtzenhain, LJ
影响因子：
9.4
作者：
CLARK, HF;HOSHINO, Y;OFFIT, PA
通讯作者：
OFFIT, PA
影响因子：
9.4
作者：
BRANDT, CD;KIM, HW;PARROTT, RH
通讯作者：
PARROTT, RH
影响因子：
3.7
作者：
Khamrin, Pattara;Maneekarn, Niwat;Ushijima, Hiroshi
通讯作者：
Ushijima, Hiroshi