Epicardium: interstitial Cajal-like cells (ICLC) highlighted by immunofluorescence.
Epicardium: interstitial Cajal-like cells (ICLC) highlighted by immunofluorescence.
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DOI：
10.1111/j.1582-4934.2009.00756.x
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发表时间：
2009-04
影响因子：
5.3
通讯作者：
Manole CG
中科院分区：
文献类型：
--
作者：
Suciu L;Popescu LM;Regalia T;Ardelean A;Manole CG
在过去的几年里，人们对心外膜在心脏发育、心肌重塑或修复和再生中的作用越来越感兴趣。在心外膜下的心外膜下的松散结缔组织中发现了几种类型的细胞。我们之前（反复）证明了间质cajal样细胞（iclc）在人类和哺乳动物的心肌中存在，无论是在心房还是在心室。在这里，我们描述了成年小鼠心外膜和原代培养以及原位冷冻切片的iclc。鉴别iclc是基于相衬显微镜和免疫表型。我们发现细胞具有独特的形态方面：纺锤形，三角形或多边形细胞体和典型的很长（几十到几百微米）和非常薄的细胞-质过程，具有独特的“串珠”外观。如活细胞的MitoTracker Green FM标记所示，扩张中含有线粒体。心外膜iclc c-kit/CD117和/或CD34阳性。然而，我们也观察到iclc中c-kit和vimentin呈阳性。总之，iclc在心内膜下代表了一种独特的细胞类型，可能至少包括两个亚群：(i) c-kit/ cd34阳性和（ii）仅c-kit阳性。iclc可能是正常和/或损伤心脏中形成心肌细胞谱系所必需的祖细胞（或启动子）。
During the last few years, there is an increasing interest in the role of the epicardium in cardiac development, myocardial remodelling or repair and regeneration. Several types of cells were described in the subepicardial loose connective tissue, beneath the epicardial mesothe-lium. We showed previously (repeatedly) the existence of interstitial Cajal-like cells (ICLCs) in human and mammalian myocardium, either in atria or in ventricles. Here, we describe ICLCs in adult mice epicardium and primary culture as well as in situ using frozen sections. The identification of ICLCs was based on phase contrast microscopy and immunophenotyping. We found cells with characteristic morphologic aspects: spindle-shaped, triangular or polygonal cell body and typical very long (tens to hundreds micrometres) and very thin cyto-plasmic processes, with a distinctive ‘beads-on-a-string’ appearance. The dilations contain mitochondria, as demonstrated by MitoTracker Green FM labelling of living cells. Epicardial ICLCs were found positive for c-kit/CD117 and/or CD34. However, we also observed ICLCs positive for c-kit and vimentin. In conclusion, ICLCs represent a distinct cell type in the subendocardium, presumably comprising at least two subpopulations: (i) c-kit/CD34-positive and (ii) only c-kit-positive. ICLCs might be essential as progenitor (or promoter) cells for developing cardiomyocyte lineages in normal and/or injured heart.
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DOI：
10.1111/j.1582-4934.2006.tb00410.x
发表时间：
2006-04
影响因子：
5.3
作者：
Popescu LM;Gherghiceanu M;Hinescu ME;Cretoiu D;Ceafalan L;Regalia T;Popescu AC;Ardeleanu C;Mandache E
通讯作者：
Mandache E
间充质干细胞和心脏修复。
影响因子：
5.3
作者：
Nesselmann C;Ma N;Bieback K;Wagner W;Ho A;Konttinen YT;Zhang H;Hinescu ME;Steinhoff G
通讯作者：
Steinhoff G
DOI：
10.1111/j.1582-4934.2006.tb00306.x
发表时间：
2006-01
影响因子：
5.3
作者：
Hinescu ME;Gherghiceanu M;Mandache E;Ciontea SM;Popescu LM
通讯作者：
Popescu LM
影响因子：
37.8
作者：
Anversa, P;Kajstura, J;Bolli, R
通讯作者：
Bolli, R
影响因子：
5.2
作者：
Castaldo, Clotilde;Di Meglio, Franca;Montagnani, Stefania
通讯作者：
Montagnani, Stefania