Performance of Ag-ELISA in the diagnosis of Taenia solium cysticercosis in naturally infected pigs in Tanzania.
Performance of Ag-ELISA in the diagnosis of Taenia solium cysticercosis in naturally infected pigs in Tanzania.
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坦桑尼亚自然感染的猪的taenia囊囊肿诊断中Ag-elisa的性能。
DOI：
10.1186/s13071-020-04416-4
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发表时间：
2020-10-27
影响因子：
3.2
通讯作者：
Ngowi HA
中科院分区：
文献类型：
--
作者：
Kabululu ML;Johansen MV;Mlangwa JED;Mkupasi EM;Braae UC;Trevisan C;Colston A;Cordel C;Lightowlers MW;Ngowi HA
猪带绦虫是一种人畜共患寄生虫，可引起神经囊尾蚴病，是人类迟发性获得性癫痫的主要原因。缺乏负担得起的、具体的和敏感的诊断工具阻碍了对寄生虫的控制。本研究评估了抗原检测酶联免疫吸附试验（Ag-ELISA）在诊断活T。坦桑尼亚流行区自然感染屠宰年龄猪的猪囊虫病。总共有350头猪在屠宰前被流血的，并检查了它们的尸体。通过使用基于单克隆抗体的B158/B60 Ag-ELISA分析血清的循环抗原。每具尸体检查有无包虫囊尾蚴，半具尸体肌肉组织连同全脑、头肌、舌、心脏和膈肌用细切口（<0.5cm）切片，以显示和计数包虫囊尾蚴。猪囊尾蚴半胴体解剖可检出至少84%的感染猪。在Stata 12中计算患病率及其95%置信区间（CI）。确定敏感性、特异性、预测值和似然比。29头猪（8.3%，95%CI：5.6-11.7%）有活T。猪囊虫病11头，猪囊虫病11头。囊虫棘球蚴感染率为3.1%，95%CI为1.6-5.5%。未观察到合并感染。68头猪（19.4%，95%CI：15.4-20%）在Ag-ELISA上检测为阳性;其中24头猪有T。猪囊尾蚴感染7例，囊尾蚴敏感性和特异性分别为82.7%和86.3%。阳性和阴性预测值分别为35.2%和98.2%。阳性和阴性Ag-ELISA试验结果的似然比分别为6.0和0.2。循环抗原滴度与T淋巴细胞密度呈显著正相关。猪囊尾蚴（r（348）= 0.63，P < 0.001）。本文报道的Ag-ELISA试验特征表明，该试验在排除T.因此，阴性结果几乎肯定表明猪没有感染，但阳性结果应始终谨慎解释。T的估计。因此，根据Ag-ELISA结果，应根据试验性能特征和T.包虫
Taenia solium is a zoonotic parasite responsible for neurocysticercosis—a major cause of late-onset acquired epilepsy in humans. Lack of affordable, specific and sensitive diagnostic tools hampers control of the parasite. This study assessed the performance of an antigen detection enzyme-linked immunosorbent assay (Ag-ELISA) in the diagnosis of viable T. solium cysticercosis in naturally infected slaughter-age pigs in an endemic area in Tanzania. A total of 350 pigs were bled before they were slaughtered and their carcases examined. Serum was analyzed for circulating antigens by using a monoclonal antibody-based B158/B60 Ag-ELISA. Each carcase was examined for the presence of Taenia hydatigena cysticerci and half carcase musculature together with the whole brain, head muscles, tongue, heart and diaphragm were sliced with fine cuts (< 0.5 cm) to reveal and enumerate T. solium cysticerci. Half carcase dissection can detect at least 84% of infected pigs. Prevalence and their 95% confidence intervals (CI) were calculated in Stata 12. Sensitivity, specificity, predictive values and likelihood ratios were determined. Twenty–nine pigs (8.3%, 95% CI: 5.6–11.7%) had viable T. solium cysticerci while 11 pigs had T. hydatigena cysticerci (3.1%, 95% CI: 1.6–5.5%). No co-infection was observed. Sixty-eight pigs (19.4%, 95% CI: 15.4–20%) tested positive on Ag-ELISA; of these, 24 had T. solium cysticerci and 7 had T. hydatigena cysticerci. Sensitivity and specificity were determined to be 82.7% and 86.3%, respectively. Positive and negative predictive values were 35.2% and 98.2%, respectively. Likelihood ratios for positive and negative Ag-ELISA test results were 6.0 and 0.2, respectively. There was a significant positive correlation between the titre of circulating antigens and intensity of T. solium cysticerci (r(348) = 0.63, P < 0.001). The Ag-ELISA test characteristics reported in this study indicate that the test is more reliable in ruling out T. solium cysticercosis in pigs, than in confirming it. Hence, a negative result will almost certainly indicate that a pig has no infection, but a positive result should always be interpreted with caution. Estimates of T. solium prevalence based on Ag-ELISA results should, therefore, be adjusted for test performance characteristics and occurrence of T. hydatigena.
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影响因子：
3.3
作者：
Muro, Claudio;Gomez-Puerta, Luis A.;O'Neal, Seth E.
通讯作者：
O'Neal, Seth E.
影响因子：
2.6
作者：
Dermauw, Veronique;Ganaba, Rasmane;Dorny, Pierre
通讯作者：
Dorny, Pierre
影响因子：
3.8
作者：
Ndimubanzi PC;Carabin H;Budke CM;Nguyen H;Qian YJ;Rainwater E;Dickey M;Reynolds S;Stoner JA
通讯作者：
Stoner JA
影响因子：
2.4
作者：
Johansen, M. V.;Trevisan, C.;Braae, U. C.
通讯作者：
Braae, U. C.
影响因子：
3.2
作者：
Chembensofu, Mwelwa;Mwape, K. E.;Gabriel, S.
通讯作者：
Gabriel, S.