A role for gamma/delta T cells in a mouse model of fracture healing.
A role for gamma/delta T cells in a mouse model of fracture healing.
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DOI：
10.1002/art.24520
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发表时间：
2009-06
影响因子：
--
通讯作者：
Tuan, Rocky S.
中科院分区：
文献类型：
--
作者：
Colburn, Nona T.;Zaal, Kristien J. M.;Wang, Francis;Tuan, Rocky S.
骨折可以引发免疫反应，通过细胞因子的产生和释放扰乱成骨细胞和破骨细胞的稳态。我们研究的目的是调查 γδ T 细胞（已知参与组织修复的先天淋巴细胞）作为骨免疫系统对体内骨损伤模型反应的潜在细胞成分。此类细胞或其效应细胞因子的缺失会影响其他应答细胞在增殖、分化、基质产生和最终愈伤组织形成方面的命运。在 60 只 γδ T 细胞受体敲除小鼠和 60 只对照 C57BL/6 小鼠中产生胫骨骨折。分析包括对照动物的 γδ 阳性子集和两组的 CD 44 的放射线照片、基本组织学、机械测试、流式细胞术和免疫组织化学定位，以及效应细胞因子、IL-2、干扰素-γ 和 IL-6 的 ELISA 分析。 γδ敲除动物表现出更成熟的组织学成分，并且在关键的修复阶段主要骨（骨唾液蛋白）和软骨（II型胶原）基质蛋白以及骨形态发生蛋白2的表达量增加。此外，只有基因敲除动物的骨祖细胞抗增殖细胞因子、IL-6 和干扰素-γ 才会减少。结果是，与对照组相比，基因敲除小鼠修复部位的稳定性得到改善，并且整体生物力学强度更佳。 γδ T 细胞在骨骼损伤中的证据证明了免疫系统对骨生物学影响的重要性，与骨免疫学领域相关，并提供了一个潜在的分子平台，可用于开发基本的治疗策略。
Fractures can initiate an immune response that disturbs osteoblastic and osteoclastic cellular homeostasis through cytokine production and release. The aim of our study was to investigate γδ T-cells, innate lymphocytes known to be involved in tissue repair, as potential cellular components of the osteoimmune’s system response to an in-vivo model of bony injury. The absence of such cells or their effector cytokines influences the fate of other responder cells in proliferation, differentiation, matrix production, and ultimate callus formation. Tibia fractures were created in 60 γδ T-cell receptor knockout and 60 control C57BL/6 mice. Analysis included radiographs, basic histology, mechanical testing, flow cytometry, and immunohistochemical localization of γδ positive subsets from control animals, and of CD 44 from both groups, as well as ELISA for the effector cytokines, IL-2, interferon-γ, and IL-6, respectively. γδ knockout animals demonstrated more mature histological elements and quantitative increases in the expression of major bone (bone sialoprotein) and cartilage (collagen type II) matrix proteins, and bone morphogenetic protein-2 at a critical reparative phase. Moreover, only knockout animals had a decrease in osteoprogenitor antiproliferative cytokines, IL-6 and interferon-γ. The result was improved stability at the repair site and an overall superior biomechanical strength in knockout mice as compared with controls. The evidence for γδ T-cells in the context of skeletal injury demonstrates the importance of the immune system’s impact on bone biology, relevant to the field of osteoimmunlogy, and offers a potential molecular platform from which to develop essential therapeutic strategies.
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影响因子：
3.1
作者：
Daniel, TanJanika;Thobe, Ljoern M.;Schwacha, Martin G.
通讯作者：
Schwacha, Martin G.
影响因子：
64.8
作者：
FERRICK, DA;SCHRENZEL, MD;LEPPER, H
通讯作者：
LEPPER, H
DOI：
10.3109/17453679408995493
发表时间：
1994-08-01
期刊：
ACTA ORTHOPAEDICA SCANDINAVICA
影响因子：
--
作者：
ANDREW, JG;ANDREW, SM;MARSH, DR
通讯作者：
MARSH, DR
影响因子：
4.4
作者：
Corthay, A;Johansson, Å;Holmdahl, R
通讯作者：
Holmdahl, R
影响因子：
1.9
作者：
GEBHARD, JS;JOHNSTONJONES, K;MEALS, RA
通讯作者：
MEALS, RA