Metagenomic sequencing characterizes a wide diversity of viruses in field mosquito samples in Nigeria.
Metagenomic sequencing characterizes a wide diversity of viruses in field mosquito samples in Nigeria.
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DOI：
10.1038/s41598-022-11797-2
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发表时间：
2022-05-10
影响因子：
4.6
通讯作者：
中科院分区：
文献类型：
--
作者：
蚊媒对公共健康构成巨大威胁。全世界六分之一的疾病是媒介传播的，主要通过蚊子传播。在过去几年中，尼日利亚许多地方都爆发了黄热病病毒（YFV）活跃疫情，全国范围内的昆虫学监测是为了了解这些疫情而做出的重大努力。在这项研究中，我们使用宏基因组测序方法来表征 2017 年至 2020 年尼日利亚多次黄热病 (YF) 爆发期间收集的载体样本中存在的病毒。蚊子样本被分为 1 至 50 只蚊子的池，每个池根据物种、性别和地点进行分组。对从 9 个州收集的 25 个伊蚊属和 1 个按蚊属进行了测序并进行了宏基因组分析。我们在该样本集中发现了属于不同科的多种病毒。检测到的七种不同病毒包括：Fako病毒、Phasi Charoen样病毒、Verdadero病毒、Chaq样病毒、埃及伊蚊病毒、细胞融合剂病毒和特萨诺伊蚊病毒。尽管没有关于这些病毒具有致病性的报道，但它们是同一科中的一个尚未被研究的群体，并且与已知的致病性虫媒病毒密切相关。我们的研究强调了下一代测序在识别昆虫特异性病毒（ISV）方面的力量，并提供了对尼日利亚蚊子载体病毒组的深入了解。
Mosquito vectors are a tremendous public health threat. One in six diseases worldwide is vector-borne transmitted mainly by mosquitoes. In the last couple of years, there have been active Yellow fever virus (YFV) outbreaks in many settings in Nigeria, and nationwide, entomological surveillance has been a significant effort geared towards understanding these outbreaks. In this study, we used a metagenomic sequencing approach to characterize viruses present in vector samples collected during various outbreaks of Yellow fever (YF) in Nigeria between 2017 and 2020. Mosquito samples were grouped into pools of 1 to 50 mosquitoes, each based on species, sex and location. Twenty-five pools of Aedes spp and one pool of Anopheles spp collected from nine states were sequenced and metagenomic analysis was carried out. We identified a wide diversity of viruses belonging to various families in this sample set. Seven different viruses detected included: Fako virus, Phasi Charoen-like virus, Verdadero virus, Chaq like-virus, Aedes aegypti totivirus, cell fusing agent virus and Tesano Aedes virus. Although there are no reports of these viruses being pathogenic, they are an understudied group in the same families and closely related to known pathogenic arboviruses. Our study highlights the power of next generation sequencing in identifying Insect specific viruses (ISVs), and provide insight into mosquito vectors virome in Nigeria.
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