The importance of corticosterone in mediating restraint-induced weight loss in rats.
The importance of corticosterone in mediating restraint-induced weight loss in rats.
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DOI：
10.1016/j.physbeh.2010.11.014
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发表时间：
2011-02-01
影响因子：
2.9
通讯作者：
Harris, Ruth B. S.
中科院分区：
文献类型：
--
作者：
Scherer, Isabel J.;Holmes, Philip V.;Harris, Ruth B. S.
关键词：
束缚3小时/天持续3天（RR）的大鼠体重减轻，并且一旦束缚结束，体重不会恢复到非束缚对照组的体重。本研究通过在每次束缚前向ADX大鼠注射2.0 mg皮质酮/kg，以复制束缚诱导的循环皮质酮激增，测试了束缚诱导的皮质酮释放在介导体重变化中的重要性。注射皮质酮的肾上腺切除（ADX）大鼠与未受限制的大鼠具有相同的初始体重减轻，而注射皮质酮的ADX大鼠和注射生理盐水的ADX大鼠的非限制性ADX大鼠的限制性注射仅产生初始体重减轻的一半。RR结束后14天测量的持续体重减轻对于注射皮质酮的束缚的完整大鼠和束缚的ADX大鼠是相同的，而注射盐水的束缚的ADX大鼠实现了与其对照相同的体重增加。皮质酮注射对未束缚的完整大鼠的体重增加没有影响。原位杂交结果显示，ADX大鼠下丘脑室旁核（PVN）中促肾上腺皮质激素释放因子（CRF）mRNA的表达与正常大鼠相同程度地增加，且不受皮质酮注射的影响。束缚、ADX或皮质酮注射对PVN精氨酸加压素（AVP）mRNA表达无明显影响。这些数据表明，皮质酮的激增导致持续的体重减轻ADX大鼠通过一种机制，可以补偿在完整的大鼠，是独立的PVN CRF或AVP mRNA表达的变化。
Rats restrained for 3 hours/day for 3 days (RR) lose weight and do not return to the weight of non-restrained controls once restraint has ended. This study tested the importance of restraint-induced corticosterone release in mediating the change in body weight by injecting ADX rats with 2.0 mg corticosterone/kg before each restraint to replicate the restraint-induced surge in circulating corticosterone. Restrained adrenalectomized (ADX) rats injected with corticosterone had the same initial weight loss as intact restrained rats, whereas corticosterone injection in non-restrained ADX rats and restraint of ADX rats injected with saline each produced only half as much initial weight loss. Sustained weight loss, measured for 14 days after the end of RR, was the same for restrained intact rats and restrained ADX rats injected with corticosterone whereas restrained ADX rats injected with saline achieved the same weight gain as their controls. Corticosterone injections had no effect on weight gain of non-restrained intact rats. In situ hybridization showed that corticotropin releasing factor (CRF) mRNA expression in the paraventricular nucleus of the hypothalamus (PVN) was increased by the same degree in ADX rats and restrained intact rats and was not modified by corticosterone injections. There was no significant effect of restraint, ADX or corticosterone injection on PVN arginine vasopressin (AVP) mRNA expression. These data indicate that a surge in corticosterone causes sustained weight loss in ADX rats through a mechanism that can be compensated for in intact rats and is independent of changes in PVN CRF or AVP mRNA expression.
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DOI：
10.1042/cs0510487
发表时间：
1976-01-01
期刊：
CLINICAL SCIENCE AND MOLECULAR MEDICINE
影响因子：
--
作者：
GREGORY, MC;DUVAL, D;MEYER, P
通讯作者：
MEYER, P
影响因子：
4.8
作者：
MAKINO, S;SCHULKIN, J;GOLD, PW
通讯作者：
GOLD, PW
影响因子：
5.2
作者：
Jankord R;Herman JP
通讯作者：
Herman JP
影响因子：
3.3
作者：
Hsu, DT;Lombardo, KA;Kalin, NH
通讯作者：
Kalin, NH
DOI：
10.1073/pnas.81.7.2006
发表时间：
1984-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
IVELL, R;RICHTER, D
通讯作者：
RICHTER, D