Drosophila R8 photoreceptor cell subtype specification requires hibris.
Drosophila R8 photoreceptor cell subtype specification requires hibris.
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DOI：
10.1371/journal.pone.0240451
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发表时间：
2020
期刊：
影响因子：
3.7
通讯作者：
Britt SG
中科院分区：
文献类型：
--
作者：
Tan H;Fulton RE;Chou WH;Birkholz DA;Mannino MP;Yamaguchi DM;Aldrich JC;Jacobsen TL;Britt SG
感觉系统中的细胞分化和细胞命运决定对于刺激辨别和环境刺激编码至关重要。色觉基于视网膜感光细胞的不同颜色敏感性，然而，控制感光细胞分化和指定颜色敏感性的发育程序却知之甚少。在果蝇中，有证据表明复眼中不同光感受器的颜色敏感性受到细胞之间的感应信号的调节，但这些信号的确切性质以及它们如何传播仍然未知。我们进行了基因筛选，以确定该过程的其他调节因子，并发现了 hibris 基因中的一个新突变，该基因编码一种非细胞识别模块蛋白（IRM）。这些免疫球蛋白超家族细胞粘附分子包括人 KIRREL 和去氧肾上腺素 (NPHS1)。 hibris 在发育中的果蝇眼睛中动态表达，功能丧失突变会产生多种突变表型，包括破坏 R8 感光细胞多样性的规范。我们证明，视网膜内需要 hibris，并且 hibris 过度表达足以破坏正常的感光细胞图案。这些发现表明，在相邻的 R7 和 R8 感光细胞中产生视蛋白基因配对表达的信号传导过程中存在额外的复杂性。
Cell differentiation and cell fate determination in sensory systems are essential for stimulus discrimination and coding of environmental stimuli. Color vision is based on the differential color sensitivity of retinal photoreceptors, however the developmental programs that control photoreceptor cell differentiation and specify color sensitivity are poorly understood. In Drosophila melanogaster, there is evidence that the color sensitivity of different photoreceptors in the compound eye is regulated by inductive signals between cells, but the exact nature of these signals and how they are propagated remains unknown. We conducted a genetic screen to identify additional regulators of this process and identified a novel mutation in the hibris gene, which encodes an irre cell recognition module protein (IRM). These immunoglobulin super family cell adhesion molecules include human KIRREL and nephrin (NPHS1). hibris is expressed dynamically in the developing Drosophila melanogaster eye and loss-of-function mutations give rise to a diverse range of mutant phenotypes including disruption of the specification of R8 photoreceptor cell diversity. We demonstrate that hibris is required within the retina, and that hibris over-expression is sufficient to disrupt normal photoreceptor cell patterning. These findings suggest an additional layer of complexity in the signaling process that produces paired expression of opsin genes in adjacent R7 and R8 photoreceptor cells.
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影响因子：
11.8
作者：
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通讯作者：
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