Attenuated Actinobacillus pleuropneumoniae double-deletion mutant S-8∆clpP/apxIIC confers protection against homologous or heterologous strain challenge.
Attenuated Actinobacillus pleuropneumoniae double-deletion mutant S-8∆clpP/apxIIC confers protection against homologous or heterologous strain challenge.
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DOI：
10.1186/s12917-016-0928-9
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发表时间：
2017-01-06
影响因子：
2.6
通讯作者：
Wang C
中科院分区：
文献类型：
--
作者：
Xie F;Li G;Zhou L;Zhang Y;Cui N;Liu S;Wang C
胸膜肺炎放线杆菌是引起猪胸膜肺炎的病原菌，给养猪业造成了巨大的经济损失。本研究对A.构建了猪胸膜肺炎疫苗，并对其安全性和保护效果进行了评价。S-8△clpP△apxIIC突变体在小鼠（BALB/c）模型中表现出减弱的毒力，即使在高达1.0 × 109 CFU的剂量下也不会对猪产生有害影响。此外，S-8△clpP△apxIIC突变体能够诱导猪产生强的免疫应答，包括高水平的IgG 1和IgG 2，刺激γ干扰素（IFN-γ）、白细胞介素12（IL-12）和白细胞介素4（IL-4）的产生，并对A.胸膜肺炎血清型7或5a。S-8△clpP△apxIIC免疫组猪攻毒后肺组织无病变，细菌载量降低。本研究结果提示，S-8△clpP△apxIIC突变体可作为一种高免疫原性的、潜在的抗A.胸膜肺炎感染
Actinobacillus pleuropneumoniae is the etiological agent of porcine pleuropneumonia, which leads to large economic losses to the swine industry worldwide. In this study, S-8△clpP△apxIIC, a double-deletion mutant of A. pleuropneumoniae was constructed, and its safety and protective efficacy were evaluated in pigs. The S-8△clpP△apxIIC mutant exhibited attenuated virulence in a murine (BALB/c) model, and caused no detrimental effects on pigs even at a dose of up to 1.0 × 109 CFU. Furthermore, the S-8△clpP△apxIIC mutant was able to induce a strong immune response in pigs, which included high levels of IgG1 and IgG2, stimulated gamma interferon (IFN-γ), interleukin 12 (IL-12), and interleukin 4 (IL-4) production, and conferred effective protection against the lethal challenge with A. pleuropneumoniae serovars 7 or 5a. The pigs in the S-8△clpP△apxIIC immunized groups have no lesions and reduced bacterial loads in the lung tissue after challenge. The data obtained in this study suggest that the S-8△clpP△apxIIC mutant can serve as a highly immunogenic and potential live attenuated vaccine candidate against A. pleuropneumoniae infection.
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影响因子：
2.4
作者：
HANNAN, PCT;BHOGAL, BS;FISH, JP
通讯作者：
FISH, JP
影响因子：
0.9
作者：
Sarkoezi, Rita;Makrai, Laszlo;Fodor, Laszlo
通讯作者：
Fodor, Laszlo
影响因子：
56.9
作者：
SNAPPER, CM;PAUL, WE
通讯作者：
PAUL, WE
影响因子：
2.1
作者：
Baltes, N;Tonpitak, W;Gerlach, GF
通讯作者：
Gerlach, GF
影响因子：
3.2
作者：
Dehio, C;Meyer, M
通讯作者：
Meyer, M