Telocytes and putative stem cells in ageing human heart.
Telocytes and putative stem cells in ageing human heart.
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DOI：
10.1111/jcmm.12509
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发表时间：
2015-01
影响因子：
5.3
通讯作者：
Gherghiceanu M
中科院分区：
文献类型：
--
作者：
Popescu LM;Curici A;Wang E;Zhang H;Hu S;Gherghiceanu M
传统认为哺乳动物心脏由约 70% 的非肌细胞（间质细胞）和 30% 的心肌细胞（CM）组成。不管怎样，自从 2010 年描述了特细胞 (TC) 以来，它们的存在一直被忽视（请访问 http://www.tel Theirs.com）。此外，人类衰老过程中心脏干细胞（CSC）的数量尚未准确估计。我们使用电子显微镜来识别和估计人类心房心肌（附件）中的细胞数量。研究了三个与年龄相关的群体：新生儿（17 天至 1 岁）、儿童（6 至 17 岁）和成人（34 至 60 岁）。使用计算机辅助技术对低倍电子显微镜图像进行形态测量。我们发现，间质面积随着年龄的增长逐渐增加，从新生儿的 31.3 ± 4.9% 到成人的 41 ± 5.2%。此外，与新生儿相比，儿童和成人的毛细血管数量（每平方毫米）增加了数百个。 CM 是数量最多的细胞，占新生儿的 76%、儿童的 88% 和成人的 86%。在 17 天的新生儿中观察到 CM 有丝分裂的图像。有趣的是，在人类新生儿和儿童的CM中没有发现脂褐质颗粒。占据间质的细胞百分比为（取决于年龄）： 内皮细胞 52-62%；血管平滑肌细胞和周细胞22-28%，神经末梢雪旺细胞6-7%，成纤维细胞3-10%，巨噬细胞1-8%，TCs约1%，干细胞不到1%。我们无法证实人们普遍认为心脏成纤维细胞是心脏中最常见的细胞类型，约占心肌体积的 20%。从数字上看，TC 代表人类心脏间质细胞的一小部分，但由于其广泛的端足，它们实现了一个 3D 网络，例如支持 CSC。心肌再生能力（非常）低的原因可能是 CSC 的数量，随着年龄的增长，CSC 的数量减少五倍（与成人相比，新生儿从 0.5% 降至 0.1%）。
Tradition considers that mammalian heart consists of about 70% non-myocytes (interstitial cells) and 30% cardiomyocytes (CMs). Anyway, the presence of telocytes (TCs) has been overlooked, since they were described in 2010 (visit http://www.telocytes.com). Also, the number of cardiac stem cells (CSCs) has not accurately estimated in humans during ageing. We used electron microscopy to identify and estimate the number of cells in human atrial myocardium (appendages). Three age-related groups were studied: newborns (17 days–1 year), children (6–17 years) and adults (34–60 years). Morphometry was performed on low-magnification electron microscope images using computer-assisted technology. We found that interstitial area gradually increases with age from 31.3 ± 4.9% in newborns to 41 ± 5.2% in adults. Also, the number of blood capillaries (per mm2) increased with several hundreds in children and adults versus newborns. CMs are the most numerous cells, representing 76% in newborns, 88% in children and 86% in adults. Images of CMs mitoses were seen in the 17-day newborns. Interestingly, no lipofuscin granules were found in CMs of human newborns and children. The percentage of cells that occupy interstitium were (depending on age): endothelial cells 52–62%; vascular smooth muscle cells and pericytes 22–28%, Schwann cells with nerve endings 6–7%, fibroblasts 3–10%, macrophages 1–8%, TCs about 1% and stem cells less than 1%. We cannot confirm the popular belief that cardiac fibroblasts are the most prevalent cell type in the heart and account for about 20% of myocardial volume. Numerically, TCs represent a small fraction of human cardiac interstitial cells, but because of their extensive telopodes, they achieve a 3D network that, for instance, supports CSCs. The myocardial (very) low capability to regenerate may be explained by the number of CSCs, which decreases fivefold by age (from 0.5% to 0.1% in newborns versus adults).
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影响因子：
2.3
作者：
Cretoiu, Sanda Maria;Radu, Beatrice Mihaela;Banciu, Adela;Banciu, Daniel Dumitru;Cretoiu, Dragos;Ceafalan, Laura Cristina;Popescu, Laurentiu Mircea
通讯作者：
Popescu, Laurentiu Mircea
影响因子：
5.3
作者：
Ceafalan L;Gherghiceanu M;Popescu LM;Simionescu O
通讯作者：
Simionescu O
DOI：
10.1530/rep-12-0369
发表时间：
2013-04
期刊：
Reproduction (Cambridge, England)
影响因子：
--
作者：
Cretoiu SM;Cretoiu D;Marin A;Radu BM;Popescu LM
通讯作者：
Popescu LM
影响因子：
--
作者：
Cretoiu, Sanda M;Popescu, Laurentiu M
通讯作者：
Popescu, Laurentiu M
DOI：
10.1152/ajpheart.00514.2007
发表时间：
2007-09-01
影响因子：
4.8
作者：
Banerjee, Indroneal;Fuseler, John W.;Baudino, Troy A.
通讯作者：
Baudino, Troy A.