Interplay between bladder microbiota and urinary antimicrobial peptides: mechanisms for human urinary tract infection risk and symptom severity.
Interplay between bladder microbiota and urinary antimicrobial peptides: mechanisms for human urinary tract infection risk and symptom severity.
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DOI：
10.1371/journal.pone.0114185
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Radek KA
中科院分区：
文献类型：
--
作者：
Nienhouse V;Gao X;Dong Q;Nelson DE;Toh E;McKinley K;Schreckenberger P;Shibata N;Fok CS;Mueller ER;Brubaker L;Wolfe AJ;Radek KA
常驻细菌群落（微生物群）和宿主抗菌肽（AMPs）都是正常宿主先天免疫反应的重要组成部分，可以限制感染和病原体诱导的炎症。然而，它们的相互依赖性尚未在尿路感染（UTI）易感性的背景下进行调查。在这里，我们探讨了泌尿微生物群和宿主AMP反应之间的相互关系，作为尿路感染风险的机制。使用前瞻性收集的女性盆底手术患者手术当日尿液标本，我们报告了特定尿液微生物群的相对丰度和/或频率区分了有或没有发生术后尿路感染的参与者。此外，尿路感染风险与特定泌尿微生物群和β-防御素AMP水平显著相关。最后，尿AMP疏水性和蛋白酶活性在发生UTI的参与者中更高，并且与UTI风险和盆底症状呈正相关。这些数据表明了尿微生物群、AMP反应和症状之间的相互依赖性，并确定了尿路感染风险的潜在机制。同时评估细菌微生物群和宿主先天免疫AMP反应可以确定某些人群中尿路感染的风险增加。
Resident bacterial communities (microbiota) and host antimicrobial peptides (AMPs) are both essential components of normal host innate immune responses that limit infection and pathogen induced inflammation. However, their interdependence has not been investigated in the context of urinary tract infection (UTI) susceptibility. Here, we explored the interrelationship between the urinary microbiota and host AMP responses as mechanisms for UTI risk. Using prospectively collected day of surgery (DOS) urine specimens from female pelvic floor surgery participants, we report that the relative abundance and/or frequency of specific urinary microbiota distinguished between participants who did or did not develop a post-operative UTI. Furthermore, UTI risk significantly correlated with both specific urinary microbiota and β-defensin AMP levels. Finally, urinary AMP hydrophobicity and protease activity were greater in participants who developed UTI, and correlated positively with both UTI risk and pelvic floor symptoms. These data demonstrate an interdependency between the urinary microbiota, AMP responses and symptoms, and identify a potential mechanism for UTI risk. Assessment of bacterial microbiota and host innate immune AMP responses in parallel may identify increased risk of UTI in certain populations.
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DOI：
10.1073/pnas.1004204107
发表时间：
2010-08-17
影响因子：
11.1
作者：
Grice, Elizabeth A.;Snitkin, Evan S.;Segre, Julia A.
通讯作者：
Segre, Julia A.
影响因子：
10.3
作者：
Clausen, M. -L.;Jungersted, J. M.;Agner, T.
通讯作者：
Agner, T.
影响因子：
6.6
作者：
Celis, JE;Rasmussen, HH;Orntoft, TF
通讯作者：
Orntoft, TF
影响因子：
7.4
作者：
Fouts DE;Pieper R;Szpakowski S;Pohl H;Knoblach S;Suh MJ;Huang ST;Ljungberg I;Sprague BM;Lucas SK;Torralba M;Nelson KE;Groah SL
通讯作者：
Groah SL
影响因子：
9.4
作者：
Arao, S;Matsuura, S;Nakanishi, H
通讯作者：
Nakanishi, H