DREADD-induced silencing of the medial amygdala reduces the preference for male pheromones and the expression of lordosis in estrous female mice.
DREADD-induced silencing of the medial amygdala reduces the preference for male pheromones and the expression of lordosis in estrous female mice.
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DOI：
10.1111/ejn.13636
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发表时间：
2017-08
期刊：
影响因子：
--
通讯作者：
Baum MJ
中科院分区：
文献类型：
--
作者：
McCarthy EA;Maqsudlu A;Bass M;Georghiou S;Cherry JA;Baum MJ
性幼稚的发情雌性小鼠寻找雄性尿信息素;然而，它们最初对雄性坐骑表现出很少的接受（脊柱前凸）行为。犁鼻-辅助嗅球对内侧杏仁核的输入调节雌性啮齿动物的求偶。我们使用可逆抑制化学发生技术（设计师受体仅由设计师药物激活; DREADDs）来评估Me信号传导对女性对男性信息素的偏好的贡献以及通常在重复测试中看到的接受性的改善。性幼稚的女性接受双侧Me注射携带抑制性DREADD的腺相关病毒。雌性动物随后切除卵巢，用卵巢激素治疗，并在腹膜内注射生理盐水或氯氮平-N-氧化物（CNO;使受感染的Me神经元超极化）后进行行为测试。CNO减弱了女性对男性和女性尿液气味的偏好。重复CNO给药也减缓了前凸的增加，否则在给予生理盐水的雌性动物中观察到。然而，当对先前用CNO处理的雌性动物给予生理盐水时，其脊柱前凸矫正率与重复给予生理盐水的其他雌性动物一样高。没有破坏性的行为影响CNO在发情期女性没有DREADD感染的ME。最后，CNO减弱的能力，男性信息素刺激Fos的表达在ME的DREADD感染的小鼠，但不是在非感染的女性。我们的研究结果肯定了Me信号在女性化学感觉偏好和脊柱前凸急性表达中的重要性。然而，他们没有提供任何迹象表明，Me信号是需要增加的感受性通常看到后，重复激素引发和测试与男性。犁鼻-辅助嗅球输入内侧杏仁核（Me）调节雌性小鼠的求爱。在试验1-5中，腹膜内注射前体药物氯氮平-N-氧化物（CNO;其使感染的神经元超极化）减少了在Me中感染抑制性DREADD的发情雌性小鼠的性接受性脊柱前凸行为。当在测试6中向先前用CNO处理的雌性动物给予生理盐水时，前凸表达与在测试1-5中重复给予生理盐水的雌性动物一样高。
Sexually naïve estrous female mice seek out male urinary pheromones; however, they initially display little receptive (lordosis) behavior in response to male mounts. Vomeronasal - accessory olfactory bulb inputs to the medial amygdala (Me) regulate courtship in female rodents. We used a reversible inhibitory chemogenetic technique (Designer Receptors Exclusively Activated by Designer Drugs; DREADDs) to assess the contribution of Me signaling to females’ preference for male pheromones and improvement in receptivity normally seen with repeated testing. Sexually naïve females received bilateral Me injections of an adeno-associated virus carrying an inhibitory DREADD. Females were later ovariectomized, treated with ovarian hormones, and given behavioral tests following intraperitoneal injections of saline or clozapine-N-oxide (CNO; which hyperpolarizes infected Me neurons). CNO attenuated females’ preference to investigate male vs female urinary odors. Repeated CNO treatment also slowed the increase in lordosis otherwise seen in females given saline. However, when saline was given to females previously treated with CNO, their lordosis quotients were as high as other females repeatedly given saline. No disruptive behavioral effects of CNO were seen in estrous females lacking DREADD infections of the Me. Finally, CNO attenuated the ability of male pheromones to stimulate Fos expression in the Me of DREADD-infected mice but not in non-infected females. Our results affirm the importance of Me signaling in females’ chemosensory preferences and in the acute expression of lordosis. However, they provide no indication that Me signaling is required for the increase in receptivity normally seen after repeated hormone priming and testing with a male. Vomeronasal-accessory olfactory bulb inputs to the medial amygdala (Me) regulate courtship in female mice. Intraperitoneal injections of the pro-drug, clozapine-N-oxide (CNO; which hyperpolarized infected neurons) over tests 1–5 reduced sexually receptive lordosis behavior in estrous female mice infected in the Me with an inhibitory DREADD. When saline was given on test 6 to females previously treated with CNO, lordosis expression was as high as in females repeatedly given saline over tests 1–5.
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DOI：
10.1073/pnas.0700293104
发表时间：
2007-03-20
影响因子：
11.1
作者：
Armbruster, Blaine N.;Li, Xiang;Roth, Bryan L.
通讯作者：
Roth, Bryan L.
影响因子：
3.3
作者：
Maras, P. M.;Petrulis, A.
通讯作者：
Petrulis, A.
影响因子：
3.3
作者：
Kang, N.;McCarthy, E. A.;Cherry, J. A.;Baum, M. J.
通讯作者：
Baum, M. J.
影响因子：
4.8
作者：
Laroche, Julie;Gasbarro, Lauren;Blaustein, Jeffrey D.
通讯作者：
Blaustein, Jeffrey D.
影响因子：
16.2
作者：
Choi, GB;Dong, HW;Anderson, DJ
通讯作者：
Anderson, DJ