Photoelectrochemical Immunosensor Array Based on Thioglycolic Acid Capped CdS Quantum Dots for Multiplexed Detection of Veterinary Drug Residues
Photoelectrochemical Immunosensor Array Based on Thioglycolic Acid Capped CdS Quantum Dots for Multiplexed Detection of Veterinary Drug Residues
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基于巯基乙酸封端的 CdS 量子点的光电化学免疫传感器阵列用于兽药残留的多重检测
DOI：
10.1002/cjoc.201100548
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发表时间：
2012-05
影响因子：
5.4
通讯作者：
Jin Litong
中科院分区：
文献类型：
--
作者：
Zhang Ningdan;Bai Jing;Xian Yuezhong;Jin Litong
研制了一种基于多电极阵列的光电化学免疫传感器，用于兽药残留的同时灵敏检测。在该体系中，聚二甲基二烯丙基氯化铵（PDDA）、Au纳米粒子（Au NPs）和巯基乙酸（TGA）修饰的CdS量子点（QD）层层组装在自制的Au电极阵列上。利用电化学阻抗谱表征了（CdS/PDDA/Au NPs/PDDA）n多层膜的组装过程。然后通过1-乙基-3-（3-二甲氨基丙基）碳二亚胺（EDC）偶联反应将克伦特罗（CB）、莱克多巴胺（RAC）和氯霉素（CAP）抗体分别共价固定在Au电极阵列上。基于CdS量子点的光电化学效应，测量了CB、RAC和CAP的浓度。在优化条件下，CB、RAC和CAP的检出限分别为25、50和2.2 pg/mL（3Δ），在猪肝样品中的回收率为95.40%~ 105.5%。结果表明，该免疫传感器阵列系统在兽药残留的实用、高效、高通量分析方面具有潜在的应用价值。
A photoelectrochemical immunosensor based on multi-electrode array was developed for simultaneous and sensitive determination of veterinary drug residues. In this system, poly(dimethyldiallylammonium chloride) (PDDA), Au nanoparticles (Au NPs) and thioglycolic acid (TGA)-capped CdS quantum dots (QDs) were layer-by-layer assembled onto the home-made Au electrode array. The assembling process of the (CdS/PDDA/Au NPs/PDDA)n multilayer was characterized by electrochemical impedance spectroscopy. And then the antibodies for clenbuterol (CB), ractopamine (RAC) and chloramphenicol (CAP) were covalently immobilized onto the Au electrode array by 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) coupling reaction, respectively. The concentrations of CB, RAC and CAP were measured based on the photoelectrochemical effects of CdS QDs. Under the optimal conditions, the limits of detection (LOD) for CB, RAC and CAP were 25, 50 and 2.2 pg/mL (3Δ), respectively, with acceptable recovery over the range of 95.40%–105.5% in pig liver samples. All results indicate that the immunosensor array system has potential application for practical, effective and high throughput analysis of veterinary drugs residues.
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