Next-generation transgenic cotton: pyramiding RNAi and Bt counters insect resistance.
Next-generation transgenic cotton: pyramiding RNAi and Bt counters insect resistance.
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DOI：
10.1111/pbi.12709
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发表时间：
2017-09
影响因子：
13.8
通讯作者：
Zhu Z
中科院分区：
文献类型：
--
作者：
Ni M;Ma W;Wang X;Gao M;Dai Y;Wei X;Zhang L;Peng Y;Chen S;Ding L;Tian Y;Li J;Wang H;Wang X;Xu G;Guo W;Yang Y;Wu Y;Heuberger S;Tabashnik BE;Zhang T;Zhu Z
从苏云金芽孢杆菌（Bt）中产生杀虫蛋白的转基因作物在世界范围内被广泛种植。为了对抗害虫对产生单一Bt毒素的作物迅速增加的抗性，转基因植物“金字塔”产生两种或更多的Bt毒素，杀死同一害虫已经被广泛采用。然而，Bt毒素之间的交叉抗性和拮抗作用限制了这种方法的可持续性。在这里，我们描述了开发和测试的第一个金字塔棉花从Bt毒素和RNA干扰（RNAi）结合保护。我们培育了两种转基因棉花植株，产生来自全球鳞翅目害虫棉铃虫的双链RNA (dsRNA)，旨在干扰其幼虫激素（JH）的代谢。我们重点研究了对JH合成至关重要的JH酸甲基转移酶（JHAMT）和JH结合蛋白（JHBP）的抑制，JH结合蛋白将JH转运到器官。2015年和2016年，我们在7种棉花上检测了一株抗Bt菌株和一株相关敏感菌株的幼虫，包括两种对照，Bt棉花，两种RNAi棉花（靶向JHAMT或JHBP）和两种金字塔（Bt棉花加每种RNAi）。两种类型的RNAi棉花对抗Bt昆虫都有效。Bt棉和RNAi对易感菌株的作用是独立的。在中国北方，数以百万计的农民种植了Bt棉花以及大量的非转基因棉蚜寄主植物，在计算机模拟条件下，与单独使用Bt棉花相比，结合了Bt毒素和RNAi的金字塔棉花大大延迟了抗性。
Transgenic crops producing insecticidal proteins from the bacterium Bacillus thuringiensis (Bt) are extensively cultivated worldwide. To counter rapidly increasing pest resistance to crops that produce single Bt toxins, transgenic plant ‘pyramids’ producing two or more Bt toxins that kill the same pest have been widely adopted. However, cross‐resistance and antagonism between Bt toxins limit the sustainability of this approach. Here we describe development and testing of the first pyramids of cotton combining protection from a Bt toxin and RNA interference (RNAi). We developed two types of transgenic cotton plants producing double‐stranded RNA (dsRNA) from the global lepidopteran pest Helicoverpa armigera designed to interfere with its metabolism of juvenile hormone (JH). We focused on suppression of JH acid methyltransferase (JHAMT), which is crucial for JH synthesis, and JH‐binding protein (JHBP), which transports JH to organs. In 2015 and 2016, we tested larvae from a Bt‐resistant strain and a related susceptible strain of H. armigera on seven types of cotton: two controls, Bt cotton, two types of RNAi cotton (targeting JHAMT or JHBP) and two pyramids (Bt cotton plus each type of RNAi). Both types of RNAi cotton were effective against Bt‐resistant insects. Bt cotton and RNAi acted independently against the susceptible strain. In computer simulations of conditions in northern China, where millions of farmers grow Bt cotton as well as abundant non‐transgenic host plants of H. armigera, pyramided cotton combining a Bt toxin and RNAi substantially delayed resistance relative to using Bt cotton alone.
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影响因子：
4.6
作者：
Jakka, Siva R. K.;Shrestha, Ram B.;Gassmann, Aaron J.
通讯作者：
Gassmann, Aaron J.
影响因子：
1.6
作者：
Furuta, Kenjiro;Ichikawa, Akio;Shiotsuki, Takahiro
通讯作者：
Shiotsuki, Takahiro
影响因子：
4.8
作者：
Asokan, R.;Chandra, G. Sharath;Sita, T.
通讯作者：
Sita, T.
影响因子：
1.7
作者：
GOULD, F
通讯作者：
GOULD, F
影响因子：
4.1
作者：
Carrière Y;Crowder DW;Tabashnik BE
通讯作者：
Tabashnik BE