Identification and Expression Analysis of G Protein-Coupled Receptors in the Miridae Insect Apolygus lucorum.
Identification and Expression Analysis of G Protein-Coupled Receptors in the Miridae Insect Apolygus lucorum.
复制标题
蝼蛄科昆虫黄鳝G蛋白偶联受体的鉴定及表达分析
DOI：
10.3389/fendo.2021.773669
复制
发表时间：
2021
影响因子：
5.2
通讯作者：
Li B
中科院分区：
文献类型：
--
作者：
Gao H;Li Y;Wang M;Song X;Tang J;Feng F;Li B
G蛋白偶联受体(GPCRs)是细胞内最大、功能最广泛的跨膜受体家族，在多种生理过程中起着重要的调节作用。斑潜蝇科(半翅目：异翅目)是昆虫中种类最多的科之一。到目前为止，关于GPCRs的信息在Miridae一直缺乏。光肩星天牛是一种世界性的杂食性害虫，对多种农作物造成严重危害，造成巨大的经济损失，是该科的代表种。通过对基因组的搜索，在灵芝中鉴定出133个GPCR。与其他模式昆虫相比，我们已经观察到在灵芝中gpr基因显著扩增，尤其是生物胺受体和神经肽受体。其中，有一个新的大分支是从已知的FMRFamide受体(FMRFaRs)复制而来的。此外，通过转录组分析确定了133个基因在不同发育阶段的时空表达谱。大多数gpr基因在灵芝整个生物体中的表达水平都很低。然而，也有少数高表达的gpr基因。LW opsins在头部的高表达可能与灵芝的夜变有关，CIRL在不同时间和不同组织中的表达表明它可能参与了灵芝的生长发育。我们还发现富含亮氨酸重复序列的C2 GPCRs(LGRs)主要分布在昆虫中的半翅目和剑翅目。本研究首次对褐飞虱的GPCRs进行了研究，为斑潜蝇科害虫的调控提供了分子靶标。
G protein-coupled receptors (GPCRs) are the largest and most versatile family of transmembrane receptors in the cell and they play a vital role in the regulation of multiple physiological processes. The family Miridae (Hemiptera: Heteroptera) is one of the most diverse families of insects. Until now, information on GPCRs has been lacking in Miridae. Apolygus lucorum, a representative species of the Miridae, is an omnivorous pest that occurs worldwide and is notorious for causing serious damage to various crops and substantial economic losses. By searching the genome, 133 GPCRs were identified in A. lucorum. Compared with other model insects, we have observed GPCR genes to be remarkably expanded in A. lucorum, especially focusing on biogenic amine receptors and neuropeptide receptors. Among these, there is a novel large clade duplicated from known FMRFamide receptors (FMRFaRs). Moreover, the temporal and spatial expression profiles of the 133 genes across developmental stages were determined by transcriptome analysis. Most GPCR genes showed a low expression level in the whole organism of A. lucorum. However, there were a few highly expressed GPCR genes. The highly expressed LW opsins in the head probably relate to nocturning of A. lucorum, and the expression of Cirl at different times and in different tissues indicated it may be involved in growth and development of A. lucorum. We also found C2 leucine-rich repeat-containing GPCRs (LGRs) were mainly distributed in Hemiptera and Phthiraptera among insects. Our study was the first investigation on GPCRs in A. lucorum and it provided a molecular target for the regulation and control of Miridae pests.
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DOI：
10.1083/jcb.150.2.f83
发表时间：
2000-07-24
期刊：
The Journal of cell biology
影响因子：
--
作者：
Brody T;Cravchik A
通讯作者：
Cravchik A
影响因子：
4.4
作者：
Antony B;Soffan A;Jakše J;Abdelazim MM;Aldosari SA;Aldawood AS;Pain A
通讯作者：
Pain A
影响因子：
64.5
作者：
Hauser AS;Chavali S;Masuho I;Jahn LJ;Martemyanov KA;Gloriam DE;Babu MM
通讯作者：
Babu MM
DOI：
10.1093/protein/7.2.195
发表时间：
1994-02-01
期刊：
PROTEIN ENGINEERING
影响因子：
--
作者：
ATTWOOD, TK;FINDLAY, JBC
通讯作者：
FINDLAY, JBC
影响因子：
3.8
作者：
Fan, Yi;Sun, Peng;Zhou, Naiming
通讯作者：
Zhou, Naiming