Oral fluid cannabinoids in chronic frequent cannabis smokers during ad libitum cannabis smoking.
Oral fluid cannabinoids in chronic frequent cannabis smokers during ad libitum cannabis smoking.
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DOI：
10.1002/dta.1718
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发表时间：
2015-06
影响因子：
2.9
通讯作者：
Huestis, Marilyn A.
中科院分区：
文献类型：
--
作者：
Lee, Dayong;Vandrey, Ryan;Mendu, Damodara R.;Murray, Jeannie A.;Barnes, Allan J.;Huestis, Marilyn A.
口腔液（OF）为临床和法医药物检测提供了一种简单，非侵入性和直接可观察的样品采集。鉴于慢性大麻吸烟者经常每天多次进行药物给药，评估随意吸烟期间大麻素的药代动力学对于分析方法的实际开发和对测试结果的知情解释非常重要。11名大麻吸烟者在一个封闭的研究单位居住了51天，并接受了4次为期5天的口服δ-9-四氢大麻酚（THC）治疗。每个给药期间隔9天，每天12：00至23：00自由吸食大麻。在最后一个自由吸烟日（研究第4、18、32和46天）的9：00-22：00采集10份OF样本。随着研究日内吸食大麻香烟数量的增加，OF THC、大麻酚（CBN）和11-去甲-9-羧基-THC（THCCOOH）也增加，自末次吸烟以来的时间（吸烟时间;范围，0.0- 17.4小时）和≥88%的检出率具有显著影响;第4天的浓度显著低于第32天和第46天的浓度，但第18天的浓度不显著低于第32天和第46天的浓度。吸烟后30分钟内，THC、CBN和THCCOOH的中位浓度分别为689µg/L、116µg/L和147 ng/L，10小时后降至19.4µg/L、2.4µg/L和87.6 ng/L。大麻二酚和11-羟基-THC的总体检出率较低，分别为29%和8.6%。大麻素处置OF的高度影响的吸食大麻的时间和组成。此外，大麻素OF浓度增加超过自由吸烟日，在增加大麻自我管理的同时，可能反映了大麻耐受性增加的发展。
Oral fluid (OF) offers a simple, non-invasive and directly observable sample collection for clinical and forensic drug testing. Given that chronic cannabis smokers often engage in drug administration multiple times daily, evaluating OF cannabinoid pharmacokinetics during ad libitum smoking is important for practical development of analytical methods and informed interpretation of test results. Eleven cannabis smokers resided on a closed research unit for 51 days, and underwent four 5-day oral delta-9-tetrahydrocannabinol (THC) treatments. Each medication period was separated by 9 days of ad libitum cannabis smoking from 12:00 to 23:00h daily. Ten OF samples were collected from 9:00–22:00h on each of the last ad libitum smoking days (Study Days 4, 18, 32, and 46). As the number of cannabis cigarettes smoked increased over study days, OF THC, cannabinol (CBN), and 11-nor-9-carboxy-THC (THCCOOH) also increased with a significant effect of time since last smoking (∆time; range, 0.0–17.4h) and ≥88% detection rates; concentrations on Day 4 were significantly lower than those on Days 32 and 46 but not Day 18. Within 30 min post smoking, median THC, CBN, and THCCOOH concentrations were 689µg/L, 116µg/L, and 147ng/L, respectively, decreasing to 19.4µg/L, 2.4µg/L, and 87.6ng/L after 10h. Cannabidiol and 11-hydroxy-THC showed overall lower detection rates of 29 and 8.6%, respectively. Cannabinoid disposition in OF was highly influenced by ∆time and composition of smoked cannabis. Furthermore, cannabinoid OF concentrations increased over ad libitum smoking days, in parallel with increased cannabis self-administration, possibly reflecting development of increased cannabis tolerance.
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影响因子：
2.5
作者：
Cone, Edward J.;Clarke, Joe;Tsanaclis, Lolita
通讯作者：
Tsanaclis, Lolita
影响因子：
3
作者：
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影响因子：
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作者：
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影响因子：
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作者：
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通讯作者：
Huestis, Marilyn A.
影响因子：
2.5
作者：
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通讯作者：
Cone, EJ