Propylenella binzhouense gen. nov., sp. nov. isolated from activated sludge, and proposal of Propylenellaceae fam. nov.
Propylenella binzhouense gen. nov., sp. nov. isolated from activated sludge, and proposal of Propylenellaceae fam. nov.
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滨州丙螺菌属
DOI：
10.1007/s10482-020-01514-2
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发表时间：
2021-01
期刊：
影响因子：
--
通讯作者：
Li Qiang
中科院分区：
文献类型：
--
作者：
Liu Yu-ling;Meng Dong;Wang Fang;Gong Xiao-fan;Gu Peng-fei;Fan Xiang-yu;Du Zong-jun;Zou Ji-dong;Li Qiang
从山东省滨州市某污水处理设施的环氧丙烷皂化废水活性污泥中分离到一株革兰氏染色阴性、不形成孢子、不运动、短棒状的好氧菌株（命名为L72T）。菌株l72t在25 ~ 40℃之间生长（30℃时生长最佳）。pH值为6.0 ~ 8.0，pH值为7.0时生长最佳。菌株l72t生长的NaCl浓度范围为0 ~ 3.0% (w/v)，最适生长范围为1.0 ~ 2.0% （w/v）。菌株l72t的主要细胞脂肪酸为C19:0cyclo ω8c、C18:1ω7c、iso-C15:0和anteiso-C15:0。菌株l72t以Q-10为主要呼吸醌。极性脂质谱由磷脂酰胆碱、糖脂、氨基磷脂、磷脂酰乙醇胺、磷脂酰丝氨酸、磷脂酰二甲基乙醇胺、一种未知脂质(L)和两种未知磷脂（PL）组成。基因组测序结果显示，其基因组大小为4,703,686 bp， G + C含量为69.0 mol%。菌株l72t与其他物种的16S rRNA基因序列相似性小于94%。基于16S rRNA基因序列和基因组数据的系统发育分析显示，菌株l72t在菌丝微生物目中形成了一个独特的系统发育谱系，将其与所有科成员分开。菌株l72t与近缘种Rhodoligotrophos defluvii的平均核苷酸一致性为70.7%，数字DNA-DNA杂交一致性为18.6%。根据表型、系统发育和化学分类资料，建立了一个新的丙烯科植物。11 .包括丙烯属（Propylenella gen. 11）和滨州丙烯属（Propylenella）。型应变为L72T（= CCTCC AB 2019081T= KCTC 72254T）。
A Gram-stain-negative, non-spore-forming, non-motile, short-rod-shaped, and aerobic bacterial strain (designated L72T) was isolated from propylene oxide saponification wastewater activated sludge obtained from a wastewater treatment facility in Binzhou (Shandong Province, PR China). Strain L72Tgrew between 25 and 40 °C (optimum growth at 30 °C). The pH range for growth was between 6.0 and 8.0 (optimum growth at pH 7.0). The range of NaCl concentrations for the growth of strain L72Twas 0-3.0 % (w/v), with optimum growth at 1.0-2.0 % (w/v). The major cellular fatty acids of strain L72Twere C19:0cyclo ω8c, C18:1ω7c, iso-C15:0, and anteiso-C15:0. Strain L72Tcontained Q-10 as the predominant respiratory quinone. The polar lipid profile was composed of Phosphatidylcholine, Glycolipid, Aminophospholipid, Phosphatidylethanolamine, Phosphatidylserine, Phosphatidyldimethylethanolamine, one unknown lipid (L) and two unidentified Phospholipids (PL). Genome sequencing revealed a genome size of 4,703,686 bp and a G + C content of 69.0 mol%. The 16S rRNA gene sequence similarities of strain L72Twith other species were less than 94%. Phylogenetic analyses based on 16S rRNA gene sequences and genome data, revealed that strain L72Tformed a distinct phylogenetic lineage within the order Hyphomicrobiales, separating them from members of all families. Strain L72Tshowed 70.7% average nucleotide identity and 18.6% digital DNA-DNA hybridization identity with the closely related species Rhodoligotrophos defluvii. Based on the phenotypic, phylogenetic and chemotaxonomic data, a new family Propylenellaceae fam. nov. comprising the genus Propylenella gen. nov. and species Propylenella binzhouense sp. nov. is proposed. The type strain is L72T(= CCTCC AB 2019081T= KCTC 72254T).
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DOI：
10.1099/ijs.0.64573-0
发表时间：
2007-04
影响因子：
2.8
作者：
Xue-Wei Xu;Yue-hong Wu;Chun-sheng Wang;A. Oren;P. Zhou;Min Wu
通讯作者：
Xue-Wei Xu;Yue-hong Wu;Chun-sheng Wang;A. Oren;P. Zhou;Min Wu
DOI：
10.1099/ijs.0.056812-0
发表时间：
2014-05-01
影响因子：
2.8
作者：
Du, Zong-Jun;Wang, Ying;Chen, Guan-Jun
通讯作者：
Chen, Guan-Jun
影响因子：
3
作者：
Meier-Kolthoff JP;Auch AF;Klenk HP;Göker M
通讯作者：
Göker M
影响因子：
3.4
作者：
Doronina, Nina V.;Poroshina, Maria N.;Trotsenko, Yuri A.
通讯作者：
Trotsenko, Yuri A.
DOI：
10.1099/ijs.0.63406-0
发表时间：
2005-05
影响因子：
2.8
作者：
Cheng-Hui Xie;A. Yokota
通讯作者：
Cheng-Hui Xie;A. Yokota