Oat chromosome and genome evolution defined by widespread terminal intergenomic translocations in polyploids.
Oat chromosome and genome evolution defined by widespread terminal intergenomic translocations in polyploids.
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DOI：
10.3389/fpls.2022.1026364
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发表时间：
2022
影响因子：
5.6
通讯作者：
中科院分区：
文献类型：
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结构染色体重排涉及易位，融合和分裂导致物种之间的进化变异和潜在的生殖隔离和基因表达的变异。虽然小麦（小麦族，禾本科）和燕麦（燕麦族）都保持基本的染色体数目x=7，燕麦的基因组显示频繁的基因组间易位，在小麦中，这些易位是相对罕见的。我们的目的是显示基因组结构多样性和基因组的关系，在四倍体，六倍体和四倍体燕麦物种和双二倍体，建立模式的基因组间易位在不同的燕麦类群使用荧光原位杂交（FISH）与四个良好表征的重复DNA序列：pAs 120，AF 226603，Ast-R171和Ast-T116。以. agadiriana（2n=4x=28），所选探针与所有染色体杂交，表明该物种起源于一个同源四倍体或具有相同基因组的近缘祖先。六倍体双倍体的基因组组成为AACCDD，而六倍体双倍体的基因组组成为AACCCCDD、AAAACCDD和AABBCCDD。燕麦的A、B、C和D基因组在参与非着丝粒、插入易位方面存在显著差异。有一个从C-到D-基因组染色体远端基因组间易位的优势。易位从A-到C-，或D-到C-基因组染色体的频率较低，证明至少有一些易位在燕麦多倍体是非相互的。罕见的易位从A-到D-，D-到A-和C-到B-基因组染色体也被可视化。基础研究对利用燕麦育种中的基因组生物多样性具有影响，这是通过从野生物种中潜在地与染色体结构相反的基因渗入，从而在双倍体亲本系中产生有害的片段重复或大缺失。
Structural chromosome rearrangements involving translocations, fusions and fissions lead to evolutionary variation between species and potentially reproductive isolation and variation in gene expression. While the wheats (Triticeae, Poaceae) and oats (Aveneae) all maintain a basic chromosome number of x=7, genomes of oats show frequent intergenomic translocations, in contrast to wheats where these translocations are relatively rare. We aimed to show genome structural diversity and genome relationships in tetraploid, hexaploid and octoploid Avena species and amphiploids, establishing patterns of intergenomic translocations across different oat taxa using fluorescence in situ hybridization (FISH) with four well-characterized repetitive DNA sequences: pAs120, AF226603, Ast-R171 and Ast-T116. In A. agadiriana (2n=4x=28), the selected probes hybridized to all chromosomes indicating that this species originated from one (autotetraploid) or closely related ancestors with the same genomes. Hexaploid amphiploids were confirmed as having the genomic composition AACCDD, while octoploid amphiploids showed three different genome compositions: AACCCCDD, AAAACCDD or AABBCCDD. The A, B, C, and D genomes of oats differ significantly in their involvement in non-centromeric, intercalary translocations. There was a predominance of distal intergenomic translocations from the C- into the D-genome chromosomes. Translocations from A- to C-, or D- to C-genome chromosomes were less frequent, proving that at least some of the translocations in oat polyploids are non-reciprocal. Rare translocations from A- to D-, D- to A- and C- to B-genome chromosomes were also visualized. The fundamental research has implications for exploiting genomic biodiversity in oat breeding through introgression from wild species potentially with contrasting chromosomal structures and hence deleterious segmental duplications or large deletions in amphiploid parental lines.
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影响因子：
4.2
作者：
通讯作者：
--
DOI：
10.3390/plants11182394
发表时间：
2022-09-14
期刊：
Plants (Basel, Switzerland)
影响因子：
--
作者：
Zhang W;Tang Z;Luo J;Li G;Yang Z;Yang M;Yang E;Fu S
通讯作者：
Fu S
DOI：
10.1098/rstb.2003.1305
发表时间：
2003-06-29
影响因子：
6.3
作者：
Comai, L;Madlung, A;Tyagi, A
通讯作者：
Tyagi, A
影响因子：
3.1
作者：
CHEN, QF;ARMSTRONG, K
通讯作者：
ARMSTRONG, K
影响因子：
3.3
作者：
Braz, Guilherme T.;He, Li;Jiang, Jiming
通讯作者：
Jiang, Jiming