NIN-like protein 8 is a master regulator of nitrate-promoted seed germination in Arabidopsis.
NIN-like protein 8 is a master regulator of nitrate-promoted seed germination in Arabidopsis.
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DOI：
10.1038/ncomms13179
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发表时间：
2016-10-12
影响因子：
16.6
通讯作者：
Nambara, Eiji
中科院分区：
文献类型：
--
作者：
Yan, Dawei;Easwaran, Vanathy;Chau, Vivian;Okamoto, Masanori;Ierullo, Matthew;Kimura, Mitsuhiro;Endo, Akira;Yano, Ryoichi;Pasha, Asher;Gong, Yunchen;Bi, Yong-Mei;Provart, Nicolas;Guttman, David;Krapp, Anne;Rothstein, Steven J.;Nambara, Eiji
种子对调节发芽的多种不同的环境刺激作出反应。硝酸盐刺激许多植物的发芽，但它是如何做到这一点的仍然不清楚。在这里，我们表明，拟南芥NIN样蛋白8（NLP 8）是必不可少的硝酸盐促进种子萌发。nlp8功能丧失突变体的种子萌发对硝酸盐没有反应。NLP 8的功能，即使在硝酸还原酶缺陷突变体的背景下，和NLP 8的要求是保守的拟南芥种质。NLP 8以硝酸盐依赖性方式降低脱落酸水平，并直接与编码脱落酸分解代谢酶的CYP707A2的启动子结合。遗传分析表明，NLP 8介导的硝酸盐促进种子萌发需要CYP707A2。最后，我们发现NLP 8定位于细胞核，与NLP 7不同，NLP 7的硝酸盐依赖性核保留似乎不会被激活，这表明种子具有独特的硝酸盐信号传导机制。 硝酸盐能刺激许多植物的种子萌发。在这里，Yan等人表明拟南芥转录因子NIN样蛋白8是响应硝酸盐刺激发芽所必需的，并诱导参与阿坝催化剂的酶的表达。
Seeds respond to multiple different environmental stimuli that regulate germination. Nitrate stimulates germination in many plants but how it does so remains unclear. Here we show that the Arabidopsis NIN-like protein 8 (NLP8) is essential for nitrate-promoted seed germination. Seed germination in nlp8 loss-of-function mutants does not respond to nitrate. NLP8 functions even in a nitrate reductase-deficient mutant background, and the requirement for NLP8 is conserved among Arabidopsis accessions. NLP8 reduces abscisic acid levels in a nitrate-dependent manner and directly binds to the promoter of CYP707A2, encoding an abscisic acid catabolic enzyme. Genetic analysis shows that NLP8-mediated promotion of seed germination by nitrate requires CYP707A2. Finally, we show that NLP8 localizes to nuclei and unlike NLP7, does not appear to be activated by nitrate-dependent nuclear retention of NLP7, suggesting that seeds have a unique mechanism for nitrate signalling. Nitrate stimulates seed germination in many plant species. Here, Yan et al. show that the Arabidopsis transcription factor NIN-like protein 8 is required to stimulate germination in response to nitrate and induces expression of an enzyme involved in ABA catabolism.
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DOI：
10.1111/tpj.12186
发表时间：
2013-06
期刊：
The Plant journal : for cell and molecular biology
影响因子：
--
作者：
Footitt S;Huang Z;Clay HA;Mead A;Finch-Savage WE
通讯作者：
Finch-Savage WE
影响因子：
11.4
作者：
Ilic, K;Berleth, T;Provart, NJ
通讯作者：
Provart, NJ
影响因子：
16.6
作者：
Marchive, Chloe;Roudier, Francois;Krapp, Anne
通讯作者：
Krapp, Anne
影响因子：
11.4
作者：
Kushiro, T;Okamoto, M;Nambara, E
通讯作者：
Nambara, E
影响因子：
1.6
作者：
Nakagawa, Tsuyoshi;Suzuki, Takarnasa;Ishiguro, Sumie
通讯作者：
Ishiguro, Sumie