Surface plasmon resonance detection using antibody-linked magnetic nanoparticles for analyte capture, purification, concentration, and signal amplification.
Surface plasmon resonance detection using antibody-linked magnetic nanoparticles for analyte capture, purification, concentration, and signal amplification.
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DOI：
10.1021/ac900007c
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发表时间：
2009-03-15
影响因子：
7.4
通讯作者：
Furlong, Clement E.
中科院分区：
文献类型：
--
作者：
Soelberg, Scott D.;Stevens, Richard C.;Limaye, Ajit P.;Furlong, Clement E.
快速、灵敏和准确地检测存在于复杂基质中的低浓度分析物是一个关键的挑战。影响许多生物传感器方案的一个问题是存在于复杂基质（如血浆、尿液、粪便和环境样本）中的干扰物的数量和性质，从而导致灵敏度和特异性的丧失。我们已经开发了一种使用抗体包被的超顺磁性纳米珠（免疫磁珠，或IMBs）从复杂基质中快速纯化、浓缩和检测目标分析物的方法。使用imb作为检测放大器时，葡萄球菌肠毒素B （SEB）的SPR检测信号显著增加。当SEB检测采用10倍浓度/纯化IMB方案时，观察到检测灵敏度显着增加。该方法成功地纯化和浓缩了血清和粪便样品中的SEB，并放大了SPR检测信号。使用该方法可以在缓冲液和粪便样本中轻松检测到浓度为100皮克/mL的SEB。通过使用两种不同的抗seb抗体，附着在磁性纳米珠上的小鼠单克隆抗体和SPR传感器表面的兔多克隆抗体，IMB协议还用于验证分析物的检测。使用相同的传感器表面，用pH值为2.2的缓冲液冲洗再生传感器表面，对粪便中的SEB进行多次检测。
Rapid, sensitive and accurate detection of analytes present in low concentrations in complex matrices is a critical challenge. One issue that affects many biosensor protocols is the number and nature of the interferents present in complex matrices such as plasma, urine, stool and environmental samples, resulting in loss of sensitivity and specificity. We have developed a method for rapid purification, concentration and detection of target analytes from complex matrices using antibody-coated superparamagnetic nanobeads (immunomagnetic beads, or IMBs). The SPR detection signal from Staphylococcal enterotoxin B (SEB) was dramatically increased when the IMBs were used as detection amplifiers. When SEB detection included a 10-fold concentration/purification IMB protocol, a substantial increase in detection sensitivity was observed. This procedure was used to successfully purify and concentrate SEB from serum and stool samples, then amplify the SPR detection signal. SEB at a concentration of 100 picograms/mL was easily detected in both buffer and stool samples using this procedure. The IMB protocol also served to verify the analyte detection by using two different anti-SEB antibodies, mouse monoclonal antibodies attached to the magnetic nanobeads and rabbit polyclonal antibodies on the SPR sensor surface. Multiple detections of SEB in stool were performed using the same sensor surface by regenerating the sensor surfaces with a pH 2.2 buffer wash.
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影响因子：
12.6
作者：
Naimushin, AN;Soelberg, SD;Furlong, CE
通讯作者：
Furlong, CE
影响因子：
6.6
作者：
Stevens, Richard C.;Soelberg, Scott D.;Furlong, Clement E.
通讯作者：
Furlong, Clement E.
用流过滤的，便携式的表面等离子体共振生物传感器系统检测唾液中皮质醇的检测。
影响因子：
7.4
作者：
Stevens, Richard C.;Soelberg, Scott D.;Near, Steve;Furlong, Clement E.
通讯作者：
Furlong, Clement E.
影响因子：
9.8
作者：
Boyce, JM;Havill, NL
通讯作者：
Havill, NL
影响因子：
11.8
作者：
Kyne, L;Hamel, MB;Kelly, CNP
通讯作者：
Kelly, CNP