Differential recovery of glycosaminoglycan after IL-1-induced degradation of bovine articular cartilage depends on degree of degradation.
Differential recovery of glycosaminoglycan after IL-1-induced degradation of bovine articular cartilage depends on degree of degradation.
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IL-1诱导的牛关节软骨降解后，糖胺聚糖的差异恢复取决于降解程度。
DOI：
10.1186/ar615
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发表时间：
2003
影响因子：
4.9
通讯作者：
Burstein, D
中科院分区：
文献类型：
--
作者：
Williams, A;Oppenheimer, RA;Gray, ML;Burstein, D
在本研究中，我们研究了il -1诱导的软骨基质耗竭后的软骨基质修复。先前的数据表明，在某些情况下，软骨细胞可以合成大分子来建立功能性的细胞外基质，以应对基质损伤或放置在基质不足的三维环境中。然而，这种“修复”发生的条件并不完全清楚。先前的研究表明，胰蛋白酶缺失的年轻牛关节软骨中的软骨细胞可以补充组织糖胺聚糖（GAG），并且在整个组织中补充的速度相对均匀，这表明所有软骨细胞具有相似的修复能力。在本研究中，我们利用IL-1暴露时基质耗竭的异质分布特征来研究耗竭的严重程度是否影响GAG补充的速率。我们采用延迟钆增强软骨磁共振成像（dGEMRIC）方法监测组织GAG浓度（[GAG]）的时空演变。对于轻度（n=4）和中度（n=10） il -1诱导的GAG缺失，我们观察到在3周的恢复期内，GAG部分恢复（分别为基线值的80%和50%）。在恢复的前2周，[GAG]以每周10-15 mg/ml的速度均匀增加。然而，在第三周内，IL-1暴露后最严重的区域的积累可以忽略不计，而那些受IL-1影响最小的区域则表现出最大的积累。这一发现可能表明，退化最严重的区域不能完全恢复，可能是因为更严重的胶原蛋白损伤；这种可能性需要进一步研究。
In the present study we examined cartilage matrix repair following IL-1-induced matrix depletion. Previous data indicated that, in some cases, chondrocytes can synthesize macromolecules to establish a functional extracellular matrix in response to a matrix-damaging insult or when placed in a three-dimensional environment with inadequate matrix. However, the conditions under which such 'repair' can occur are not entirely clear. Prior studies have shown that chondrocytes in trypsin-depleted young bovine articular cartilage can replenish tissue glycosaminoglycan (GAG) and that the rate of replenishment is relatively uniform throughout the tissue, suggesting that all chondrocytes have similar capacity for repair. In the present study we used the characteristic heterogeneous distribution of matrix depletion in response to IL-1 exposure in order to investigate whether the severity of depletion influenced the rate of GAG replenishment. We used the delayed Gadolinium-Enhanced Magnetic Resonance Imaging of Cartilage (dGEMRIC) method to monitor the spatial and temporal evolution of tissue GAG concentration ([GAG]). For both mild (n=4) and moderate (n=10) IL-1-induced GAG depletion, we observed partial recovery of GAG (80% and 50% of baseline values, respectively) over a 3-week recovery period. During the first 2 weeks of recovery, [GAG] increased homogeneously at 10–15 mg/ml per week. However, during the third week the regions most severely depleted following IL-1 exposure showed negligible [GAG] accumulation, whereas those regions affected the least by IL-1 demonstrated the greatest accumulation. This finding could suggest that the most severely degraded regions do not recover fully, possibly because of more severe collagen damage; this possibility requires further examination.
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影响因子：
6.9
作者：
Potter, K;Butler, JJ;Spencer, RGS
通讯作者：
Spencer, RGS
影响因子：
2.8
作者：
Bonassar, LJ;Grodzinsky, AJ;Trippel, SB
通讯作者：
Trippel, SB
影响因子：
1.4
作者：
Gray, M L;Burstein, D;Xia, Y
通讯作者：
Xia, Y
影响因子：
--
作者：
KRUIJSEN, MWM;VANDENBERG, WB;VANDEPUTTE, LBA
通讯作者：
VANDEPUTTE, LBA
影响因子：
27.4
作者：
THOMAS, DPP;KING, B;DINGLE, JT
通讯作者：
DINGLE, JT