Prevalence, serotype diversity, biofilm-forming ability and eradication of Listeria monocytogenes isolated from diverse foods in Shanghai, China
Prevalence, serotype diversity, biofilm-forming ability and eradication of Listeria monocytogenes isolated from diverse foods in Shanghai, China
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中国上海多种食品中单核细胞增生李斯特菌的流行率、血清型多样性、生物膜形成能力和根除情况
DOI：
10.1016/j.foodcont.2016.10.025
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发表时间：
2017-03
期刊：
影响因子：
6
通讯作者：
Shi Xianming
中科院分区：
文献类型：
--
作者：
Wang Wenkai;Zhou Xiujuan;Suo Yujuan;Deng Xiangyu;Cheng Mengya;Shi Chunlei;Shi Xianming
单核细胞增生性李斯特菌是一种主要的食源性致病菌，以其在人类感染后的高死亡率而闻名。本研究旨在调查不同食物中产单核细胞李氏杆菌的流行情况和血清型多样性。中国，2013年7月至2014年6月，从上海露天市场、大型超市、熟食店和奶牛场共采集了648份食品样品，来自生奶、速冻食品、蔬菜、生肉、熟肉、水产品、乳制品和豆制品。经细菌学和分子生物学方法证实，有40例(6.2%)单核细胞增多症标本呈阳性。在调查的食品类别中，生肉样本的感染率最高，为16.0%(15/94)，其次是速冻食品样本的9.9%(10/101)和熟肉样本的7.3%(4/55)。血清学分型以1/2a型为主(67.5%)，其次为1/2b(22.5%)、1/2c(7.5%)和3c(2.5%)。对分离株形成生物膜的能力进行了评估。不同储存温度和不同血清型的细菌在生物膜形成能力上存在显著差异。通过测定生物膜中单核细胞生成的最低有效浓度(MEC)，考察了工业消毒剂二氧化氯(ClO2)和过氧化氢(H_2O_2)的抗生物被膜效果。生物膜对H_2O_2的抗性与生物膜的生物量呈正相关。ClO2对生物被膜有较强的杀灭作用，能灭活生物被膜状态下的所有李斯特菌细胞，而按中国食品安全标准推荐的浓度，过氧化氢不能杀灭生物被膜中的所有细胞。我们的研究表明，上海地区存在潜在的单核细胞增多性李斯特菌感染的公共卫生风险。在评价消毒剂时，应考虑对产单核细胞生物被膜的疗效。
Listeria monocytogenesis a major foodborne pathogen that is well known for its high mortality rate in humans upon infection. This study aimed to investigate the prevalence and serotype diversity ofL. monocytogenesisolates in various foods. From July 2013 to June 2014, a total of 648 food samples, originating from raw milk, quick-frozen food, vegetables, raw meats, cooked meats, aquatic products, dairy products and bean products, were collected from open-air markets, large supermarkets, delis and dairy farms, in Shanghai, China. Forty samples (6.2%) tested positive forL. monocytogenes, confirmed by both bacteriological and molecular methods. Among the food categories surveyed, the highest prevalence was observed in raw meat samples at 16.0% (15/94), followed by 9.9% (10/101) in quick-frozen food samples and 7.3% (4/55) in cooked meat samples. Serotyping showed that majority of the isolates (67.5%) belonged to serotype 1/2a, followed by 1/2b (22.5%), 1/2c (7.5%) and 3c (2.5%). Isolates were assessed for their ability to form biofilms. Significant differences in biofilm forming abilities were observed between storage temperatures and serotypes. Anti-biofilm efficacy of industrial disinfectants, chlorine dioxide (ClO2) and hydrogen peroxide (H2O2), was examined by measuring minimal effective concentration (MEC) onL. monocytogenesin biofilm. A positive correlation was observed among the biofilm’s resistance to H2O2and the biomass of biofilms. ClO2was found to be more effective against biofilm and inactivated all the listerial cells in the biofilm state, whereas H2O2was not able to kill all the cells in biofilms at the recommended concentrations according to National Food Safety Standard of China. Our study suggests that there is potential public health risk ofL. monocytogenesinfection in Shanghai. Efficacy againstL. monocytogenesbiofilm should be considered when evaluating disinfectants.
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