Sex-dependent differences in the gut microbiota following chronic nasal inflammation in adult mice.
Sex-dependent differences in the gut microbiota following chronic nasal inflammation in adult mice.
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DOI：
10.1038/s41598-021-83896-5
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发表时间：
2021-02-25
影响因子：
4.6
通讯作者：
Hasegawa-Ishii S
中科院分区：
文献类型：
--
作者：
Mishima Y;Osaki T;Shimada A;Kamiya S;Hasegawa-Ishii S
越来越多的证据表明嗅觉功能障碍和精神障碍的发病机制之间的关系。我们以往的研究表明，慢性鼻腔炎症可导致嗅觉感觉神经元的丢失和嗅球的萎缩，这可能导致嗅觉功能障碍。同时，越来越多的报告阐明了肠道微生物群对维持大脑功能的重要性，以及生态失调可能与神经精神疾病有关。在这里，我们研究了慢性鼻腔炎症是否会扰乱肠道微生物群，以及这种模式是否存在性别差异。8周龄C57 BL/6小鼠每周3次重复接受双侧鼻腔给予脂多糖（LPS）以引起慢性鼻腔炎症或盐水作为对照。在9周时，盲肠粪便用于16 S宏基因组分析，全血和新鲜脾组织用于ELISA分析。微生物群分析表明，慢性鼻腔炎症雄性小鼠肠道微生物群发生了显著变化，这与雌性小鼠不同。在这两种小鼠中，均未发生全身性炎症。这首次表明慢性鼻腔炎症与肠道微生物群的性别依赖性变化相关。其具体机制和对脑功能的潜在影响有待进一步研究。
A growing body of evidence suggests a relationship between olfactory dysfunction and the pathogenesis of mental disorders. Our previous studies indicated that chronic nasal inflammation caused loss of olfactory sensory neurons and gross atrophy of the olfactory bulb, which may lead to olfactory dysfunction. Simultaneously, increasing numbers of reports have elucidated the importance of gut microbiota to maintain brain function and that dysbiosis may be associated with neuropsychiatric disorders. Here we examined whether chronic nasal inflammation perturbed gut microbiota and whether there were sex differences in this pattern. Eight-week-old C57BL/6 mice repeatedly received bilateral nasal administration of lipopolysaccharide (LPS) 3 times/week to cause chronic nasal inflammation or saline as a control. At 9 weeks, cecal feces were used for 16S metagenomic analysis and whole blood and fresh tissue of spleen were used for ELISA analyses. Microbiome analysis demonstrated a remarkable change of the gut microbiota in male mice with chronic nasal inflammation which was different from that in female mice. In both mice, systemic inflammation did not occur. This has shown for the first time that chronic nasal inflammation correlates with sex-dependent changes in the gut microbiota. The detailed mechanism and potential alteration to brain functions await further studies.
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影响因子：
4.6
作者：
Jang HM;Lee KE;Lee HJ;Kim DH
通讯作者：
Kim DH
影响因子：
7.3
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影响因子：
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