A Light-Triggered Synthetic Nanopore for Controlling Molecular Transport Across Biological Membranes.
A Light-Triggered Synthetic Nanopore for Controlling Molecular Transport Across Biological Membranes.
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DOI：
10.1002/anie.202210886
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发表时间：
2022-12-23
影响因子：
16.6
通讯作者：
Howorka, Stefan
中科院分区：
文献类型：
--
作者：
Offenbartl-Stiegert, Daniel;Rottensteiner, Alexia;Dorey, Adam;Howorka, Stefan
利用光控制生物分子过程在生物研究和生物医学中很有意义，因为光可以以非侵入性和无毒的方式进行精确和选择性的激活。受益于光控制的分子过程是跨生物膜的货物运输，这通常通过膜穿刺桶形纳米孔来实现。然而，在构建更复杂的门控孔方面也有相当大的收获。在这里，我们开创了一种合成的光门纳米结构，它通过可控的盖子调节跨膜运输。光触发纳米孔是由六个成孔DNA链和一个携带光可切换偶氮苯分子的盖链自组装而成的。暴露于光打开孔，以允许小分子跨膜运输。我们的光触发孔推进了仿生化学和DNA纳米技术，可用于生物技术、生物传感、靶向药物释放或合成细胞。合成的光门控纳米孔控制分子穿过双层膜的运输。孔自组装从七个DNA链，包括一个盖子链携带光开关偶氮苯生色团。门控孔可用于研究、生物传感和靶向药物释放。
Controlling biological molecular processes with light is of interest in biological research and biomedicine, as light allows precise and selective activation in a non‐invasive and non‐toxic manner. A molecular process benefitting from light control is the transport of cargo across biological membranes, which is conventionally achieved by membrane‐puncturing barrel‐shaped nanopores. Yet, there is also considerable gain in constructing more complex gated pores. Here, we pioneer a synthetic light‐gated nanostructure which regulates transport across membranes via a controllable lid. The light‐triggered nanopore is self‐assembled from six pore‐forming DNA strands and a lid strand carrying light‐switchable azobenzene molecules. Exposure to light opens the pore to allow small‐molecule transport across membranes. Our light‐triggered pore advances biomimetic chemistry and DNA nanotechnology and may be used in biotechnology, biosensing, targeted drug release, or synthetic cells. A synthetic light‐gated nanopore controls transport of molecules across bilayer membranes. The pore self‐assembles from seven DNA strands including one lid strand carrying light‐switchable azobenzene chromophores. The gated pore may be used for research, biosensing, and targeted drug release.
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影响因子：
16.6
作者：
Burns, Jonathan R.;Goepfrich, Kerstin;Wood, James W.;Thacker, Vivek V.;Stulz, Eugen;Keyser, Ulrich F.;Howorka, Stefan
通讯作者：
Howorka, Stefan
影响因子：
16.6
作者：
Burns, Jonathan R.;Al-Juffali, Noura;Janes, Sam M.;Howorka, Stefan
通讯作者：
Howorka, Stefan
影响因子：
46.2
作者：
Darrah KE;Deiters A
通讯作者：
Deiters A
影响因子：
25
作者：
Boyden, ES;Zhang, F;Deisseroth, K
通讯作者：
Deisseroth, K
影响因子：
38.3
作者：
Burns, Jonathan R.;Seifert, Astrid;Howorka, Stefan
通讯作者：
Howorka, Stefan