A Solanum neorickii introgression population providing a powerful complement to the extensively characterized Solanum pennellii population.
A Solanum neorickii introgression population providing a powerful complement to the extensively characterized Solanum pennellii population.
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DOI：
10.1111/tpj.14095
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发表时间：
2019-01
期刊：
影响因子：
--
通讯作者：
Fernie AR
中科院分区：
文献类型：
--
作者：
Brog YM;Osorio S;Yichie Y;Alseekh S;Bensal E;Kochevenko A;Zamir D;Fernie AR
我们提出了一个番茄性状精细定位的补充资源，这些资源基于栽培番茄和野生番茄物种Solanum pennellii之间的种内杂交，在过去的20年中，这些资源已被广泛用于番茄的数量遗传学。目前的回交自交系（BILs）群体由野生物种Solanum neorickii（LA 2133）和栽培番茄（栽培品种TA 209）的后代三次回交后衍生的107个品系组成，并可免费提供给科学界。利用10 K SolCAP单核苷酸多态性芯片对这些新立克次体BIL进行基因分型，并利用3111个多态性标记定位相对于番茄物理图谱的重组断裂点。BIL平均每个系有4.3个基因渗入，平均基因渗入长度为34.7 Mbp，允许将基因组划分为340个箱，从而促进快速性状作图。我们展示了使用这种资源的力量与档案数据进行比较S. pennellii资源的代谢数量性状基因座分析后，从S. neorickii BIL收获的水果的气相色谱-质谱。然后在F2群体中并通过基于农杆菌渗入的过表达测试和验证代谢候选基因苯丙氨酸解氨酶和胱硫醚γ-裂解酶，以验证该方法在鉴定驱动番茄代谢表型的基因中的保真度。在这里，我们提出了一个互补的发展人口来自杂交野生物种Solanum neorickii（LA 2133）与栽培番茄（品种TA 209）使用测序回交。该群体由107个品系（回交自交系，BILs）组成，具有3111个多态性标记，用于绘制相对于番茄物理图谱的重组断点。此外，我们还对采自新瑞氏番茄BIL的果实进行了代谢数量性状位点分析。对代谢候选基因苯丙氨酸解氨酶和胱硫醚γ-裂解酶进行了鉴定和验证。
We present a complementary resource for trait fine‐mapping in tomato to those based on the intra‐specific cross between cultivated tomato and the wild tomato species Solanum pennellii, which have been extensively used for quantitative genetics in tomato over the last 20 years. The current population of backcross inbred lines (BILs) is composed of 107 lines derived after three backcrosses of progeny of the wild species Solanum neorickii (LA2133) and cultivated tomato (cultivar TA209) and is freely available to the scientific community. These S. neorickii BILs were genotyped using the 10K SolCAP single nucleotide polymorphism chip, and 3111 polymorphic markers were used to map recombination break points relative to the physical map of Solanum lycopersicum. The BILs harbor on average 4.3 introgressions per line, with a mean introgression length of 34.7 Mbp, allowing partitioning of the genome into 340 bins and thereby facilitating rapid trait mapping. We demonstrate the power of using this resource in comparison with archival data from the S. pennellii resources by carrying out metabolic quantitative trait locus analysis following gas chromatography–mass spectrometry on fruits harvested from the S. neorickii BILs. The metabolic candidate genes phenylalanine ammonia‐lyase and cystathionine gamma‐lyase were then tested and validated in F2 populations and via agroinfiltration‐based overexpression in order to exemplify the fidelity of this method in identifying the genes that drive tomato metabolic phenotypes. Here we present a complementary developed population derived from crossing the wild species Solanum neorickii (LA2133) with cultivated tomato (cultivar TA209) using sequenced backcrosses. The population is composed of 107 lines (backcross inbred lines, BILs) with 3111 polymorphic markers used to map recombination break points relative to the physical map of Solanum lycopersicum. In addition we carried out metabolic quantitative trait locus analysis on fruits harvested from the S. neorickii BILs. The metabolic candidate genes phenylalanine ammonia‐lyase and cystathionine gamma‐lyase were identified and validated.
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新的先进的番茄番茄种群可以实现高分辨率的叶子QTL映射和基因识别。
DOI：
10.1534/g3.116.030536
发表时间：
2016-10-13
期刊：
G3 (Bethesda, Md.)
影响因子：
--
作者：
Fulop D;Ranjan A;Ofner I;Covington MF;Chitwood DH;West D;Ichihashi Y;Headland L;Zamir D;Maloof JN;Sinha NR
通讯作者：
Sinha NR
影响因子：
6.9
作者：
Kochevenko A;Fernie AR
通讯作者：
Fernie AR
影响因子：
5.4
作者：
Gur, Amit;Osorio, Sonia;Fridman, Eyal;Fernie, Alisdair R.;Zamir, Dani
通讯作者：
Zamir, Dani
影响因子：
9.4
作者：
Bauchet, Guillaume;Grenier, Stephane;Causse, Mathilde
通讯作者：
Causse, Mathilde
影响因子：
7.4
作者：
Do, Phuc Thi;Prudent, Marion;Fernie, Alisdair R.
通讯作者：
Fernie, Alisdair R.