Alleviating the toxicity concerns of antibacterial cinnamon-polycaprolactone biomaterials for healthcare-related biomedical applications.
Alleviating the toxicity concerns of antibacterial cinnamon-polycaprolactone biomaterials for healthcare-related biomedical applications.
复制标题
减轻抗菌肉桂 - 聚己内酯生物材料在医疗相关生物医学应用中的毒性问题。
作者：
Ahmed J;Gultekinoglu M;Bayram C;Kart D;Ulubayram K;Edirisinghe M
先前已表明，含有肉桂提取物的纤维结构具有强大的抗真菌能力。问题仍然在于这些结构在纤维形式下对预防细菌感染是否有用，以及抗菌效果对于天然生理细胞的毒性意味着什么。在这项工作中，通过加压旋转成功制备了含有肉桂提取物的聚（ε - 己内酯）（PCL）纤维，其平均尺寸约为2μm。对含有肉桂提取物的PCL纤维针对大肠杆菌、金黄色葡萄球菌、耐甲氧西林金黄色葡萄球菌和粪肠球菌等细菌种类进行了抗菌能力测试；发现这些纤维能够使细菌种类的活细胞数量比对照组减少多达两个数量级。通过扫描电子显微镜（SEM）评估抗菌测试的结果。这些结构还在间接MTT测试下进行了测试，结果显示它们几乎没有毒性，与对照组相似。此外，细胞活力荧光成像显示，即使在最高测试浓度下，纤维也没有明显的毒性问题。在此，我们提出了一种可行的方法，用于生产无毒且天然丰富的肉桂提取物纤维，可用于众多生物医学应用。
将肉桂直接提取到聚己内酯聚合物基质中，通过加压旋转纺丝，生产出高产率的类似绷带的肉桂提取物纤维。然后对这些纤维进行表征，并针对大肠杆菌、金黄色葡萄球菌、耐甲氧西林金黄色葡萄球菌和粪肠球菌进行细胞毒性和抗菌活性测试。
Fibrous constructs with incorporated cinnamon‐extract have previously been shown to have potent antifungal abilities. The question remains to whether these constructs are useful in the prevention of bacterial infections in fiber form and what the antimicrobial effects means in terms of toxicity to the native physiological cells. In this work, cinnamon extract containing poly (ε‐caprolactone) (PCL) fibers were successfully manufactured by pressurized gyration and had an average size of ∼2 μm. Cinnamon extract containing PCL fibers were tested against Escherichia coli, Staphylococcus aureus, Methicillin resistant staphylococcus aureus, and Enterococcus faecalis bacterial species to assess their antibacterial capacity; it was found that these fibers were able to reduce viable cell numbers of the bacterial species up to two orders of magnitude lower than the control group. The results of the antibacterial tests were assessed by scanning electron microscopy (SEM). The constructs were also tested under indirect MTT tests where they showed little to no toxicity, similar to the control groups. Additionally, cell viability fluorescent imaging displayed no significant toxicity issues with the fibers, even at their highest tested concentration. Here we present a viable method for the production the non‐toxic and naturally abundant cinnamon extracted fibers for numerous biomedical applications. Cinnamon directly extracted into a polycaprolactone polymer base was spun with pressurized gyration to produce a high yield of cinnamon‐extracted bandage‐like fibers. These fibers where then characterized and tested for cytotoxity and antibacterial activity against Escherichia coli, Staphylococcus aureus, Methicillin Resistant Staphylococcus aureus and Enterococcus faecalis.
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影响因子：
14
作者：
Bashur, Chris A.;Dahlgren, Linda A.;Goldstein, Aaron S.
通讯作者：
Goldstein, Aaron S.
DOI：
10.1016/j.msec.2012.12.015
发表时间：
2013-04-01
影响因子：
7.9
作者：
Gautam, Sneh;Dinda, Amit Kumar;Mishra, Narayan Chandra
通讯作者：
Mishra, Narayan Chandra
影响因子：
1.6
作者：
Bang, KH;Lee, DW;Rhee, YH
通讯作者：
Rhee, YH
影响因子：
4.3
作者：
Hooth, MJ;Sills, RC;Bucher, JR
通讯作者：
Bucher, JR
影响因子：
8.4
作者：
Ambrosioni, Juan;Bouchuiguir-Wafa, Kalthum;Garbino, Jorge
通讯作者：
Garbino, Jorge