Recombinant Newcastle disease virus (NDV) expressing Duck Tembusu virus (DTMUV) pre-membrane and envelope proteins protects ducks against DTMUV and NDV challenge.
Recombinant Newcastle disease virus (NDV) expressing Duck Tembusu virus (DTMUV) pre-membrane and envelope proteins protects ducks against DTMUV and NDV challenge.
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DOI：
10.1016/j.vetmic.2018.03.027
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发表时间：
2018-05
影响因子：
3.3
通讯作者：
Zhang C
中科院分区：
文献类型：
--
作者：
Sun M;Dong J;Li L;Lin Q;Sun J;Liu Z;Shen H;Zhang J;Ren T;Zhang C
首先制备了表达DTMUV膜前蛋白和包膜蛋白的ndv载体鸭坦布苏病毒（DTMUV）二价疫苗。评价ndv载体鸭坦布苏病毒二价疫苗的有效性。为水禽NDV和DTMUV的防治提供了新的方法。自2010年以来，新出现的鸭坦布苏病毒（DTMUV）造成了中国水禽养殖区相当大的经济损失。同时，新城疫病毒（NDV）也在水禽中引起零星爆发。目前有针对这两种疾病的单独疫苗，但没有针对这两种疾病的双价疫苗或联合疫苗。在这里，我们构建了一种重组ndv载体候选疫苗，表达DTMUV的膜前（prM）和包膜(E)基因，命名为aGM/prM + E。外源prM和E蛋白在aGM/prM + E中稳定表达，表现出与亲本病毒相似的致病性和更高的生长动力学。aGM/prM + E携带一个融合切割位点，与经常从水禽中分离的无毒病毒一致，并诱导显著（p < 0.001）比市售NDV活疫苗（LaSota株）更高的NDV特异性血凝抑制（HI）滴度。与市售的DTMUV灭活疫苗（HB株）相比，aGM/prM + E的病毒中和（VN）滴度也显著提高（p < 0.05）。aGM/prM + E不仅对NDV具有完全的保护作用，而且减少了卵巢卵泡的总损伤，对鸭的DTMUV具有80%的保护作用。我们注意到aGM/prM + E疫苗可以防止攻毒鸭的NDV和DTMUV脱落。结果表明，候选疫苗aGM/prM + E有助于减少中国水禽养殖区NDV和DTMUV的传播。
Firstly generated a NDV-vectored Duck Tembusu Virus (DTMUV) bivalent vaccine that expressing the pre-membrane and envelope proteins of DTMUV. Evaluated the efficacy of the NDV-vectored Duck Tembusu Virus bivalent vaccine. Provided a new method for NDV and DTMUV controlling in waterfowl. The newly emerged Duck Tembusu virus (DTMUV) is responsible for considerable economic loss in waterfowl-raising areas in China since 2010. Meanwhile, the virulent Newcastle disease virus (NDV) has also caused sporadic outbreaks in waterfowl. The individual vaccines against both diseases are available, however, there is no bivalent or combined vaccine for either disease. Here, we constructed a recombinant NDV-vectored vaccine candidate that expresses the pre-membrane (prM) and envelope (E) genes from DTMUV, designated as aGM/prM + E. The foreign prM and E proteins were stably expressed in aGM/prM + E and exhibited similar pathogenicity but higher growth kinetics than those of the parental virus. The aGM/prM + E carries a fusion cleavage site in accordance with avirulent viruses that have been frequently isolated from waterfowl, and induced remarkably (p < 0.001) higher NDV-specific hemagglutination inhibition (HI) titers than commercially available live NDV vaccines (LaSota strain). The aGM/prM + E also elicited significantly higher (p < 0.05) virus neutralization (VN) titers than commercially available DTMUV inactivated vaccines (HB strain). The aGM/prM + E not only provided complete protection against NDV challenge but also reduced the gross lesions on ovarian folliculi and provided 80% protection against DTMUV in ducks. We note that the aGM/prM + E vaccine can prevent challenged ducks from shedding of NDV and DTMUV. Our results suggest that the candidate vaccine aGM/prM + E would help decrease NDV and DTMUV transmissions in waterfowl raising areas in China.
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影响因子：
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作者：
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通讯作者：
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影响因子：
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