An Intronless β-amyrin Synthase Gene is More Efficient in Oleanolic Acid Accumulation than its Paralog in Gentiana straminea.
An Intronless β-amyrin Synthase Gene is More Efficient in Oleanolic Acid Accumulation than its Paralog in Gentiana straminea.
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Intronless β-amyrin 合酶基因在齐墩果酸积累方面比其 Paralog 在 Gentiana straminea 中更有效
DOI：
10.1038/srep33364
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发表时间：
2016-09-14
影响因子：
4.6
通讯作者：
Xiang F
中科院分区：
文献类型：
--
作者：
Liu Y;Zhao Z;Xue Z;Wang L;Cai Y;Wang P;Wei T;Gong J;Liu Z;Li J;Li S;Xiang F
Paralogous members of the oxidosqualene cyclase (OSC) family encode a diversity of enzymes that are important in triterpenoid biosynthesis. This report describes the isolation of the Gentiana straminea gene GsAS2 that encodes a β-amyrin synthase (βAS) enzyme. Unlike its previously isolated paralog GsAS1, GsAS2 lacks introns. Its predicted protein product was is a 759 residue polypeptide that shares high homology with other known β-amyrin synthases (βASs). Heterologously expressed GsAS2 generates more β-amyrin in yeast than does GsAS1. Constitutive over-expression of GsAS2 resulted in a 5.7 fold increase in oleanolic acid accumulation, while over-expression of GsAS1 led to a 3 fold increase. Additionally, RNAi-directed suppression of GsAS2 and GsAS1 in G. straminea decreased oleonolic acid levels by 65.9% and 21% respectively, indicating that GsAS2 plays a more important role than GsAS1 in oleanolic acid biosynthesis in G. straminea. We uses a docking model to explore the catalytic mechanism of GsAS1/2 and predicted that GsAS2, with its Y560, have higher efficiency than GsAS1 and mutated versions of GsAS2 in β-amyrin produce. When the key residue in GsAS2 was mutagenized, it produced about 41.29% and 71.15% less β-amyrin than native, while the key residue in GsAS1 was mutagenized to that in GsAS2, the mutant produced 38.02% more β-amyrin than native GsAS1.
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影响因子：
3.5
作者：
Huang Z;Lin J;Cheng Z;Xu M;Guo M;Huang X;Yang Z;Zheng J
通讯作者：
Zheng J
DOI：
10.1038/nrg2487
发表时间：
2009-01
期刊：
Nature reviews. Genetics
影响因子：
--
作者：
通讯作者：
--
影响因子：
3.8
作者：
Kajikawa, M;Yamato, KT;Ohyama, K
通讯作者：
Ohyama, K
影响因子：
5.1
作者：
Iturbe-Ormaetxe, I;Haralampidis, K;Osbourn, AE
通讯作者：
Osbourn, AE
影响因子：
3
作者：
Karnik A;Karnik R;Grefen C
通讯作者：
Grefen C