Mechanism and regulation of rapid telomere prophase movements in mouse meiotic chromosomes.
Mechanism and regulation of rapid telomere prophase movements in mouse meiotic chromosomes.
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DOI：
10.1016/j.celrep.2015.03.045
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发表时间：
2015-04-28
期刊：
影响因子：
8.8
通讯作者：
Pezza RJ
中科院分区：
文献类型：
--
作者：
Lee CY;Horn HF;Stewart CL;Burke B;Bolcun-Filas E;Schimenti JC;Dresser ME;Pezza RJ
端粒引导的减数分裂前期快速前期运动（RPM）已在多种真核生物中观察到。RPM的一个共同特征是驱动染色体运动的力从细胞骨架通过核膜传递到端粒。在小鼠中的研究表明，动力蛋白沿着沿着微管的运动通过SUN 1/KASH 5核膜桥传递到端粒以产生RPM。我们使用四维荧光成像和定量运动分析来监测小鼠曲细精管中的RPM，以表征RPM过程中的运动模式。我们发现，RPM反映了核旋转和个别染色体运动的组合。端粒沿着微管轨迹运动，这些轨迹与细胞骨架网络明显连续，并在前期的不同阶段表现出特征性的排列。定量测量证实，SUN 1/KASH 5，微管，动力蛋白，但不是肌动蛋白是必需的RPM和减数分裂重组和突触的缺陷导致改变RPM。
Telomere-led rapid prophase movements (RPMs) in meiotic prophase have been observed in diverse eukaryote species. A shared feature of RPMs is that the force that drives the chromosomal movements is transmitted from the cytoskeleton, through the nuclear envelope, to the telomeres. Studies in mice suggested that dynein movement along microtubules is transmitted to telomeres through SUN1/KASH5 nuclear envelope bridges to generate RPMs. We monitored RPMs in mouse seminiferous tubules using four-dimensional fluorescence imaging and quantitative motion analysis to characterize patterns of movement in the RPM process. We find that RPMs reflect a combination of nuclear rotation and individual chromosome movements. The telomeres move along microtubule tracks which are apparently continuous with the cytoskeletal network, and exhibit characteristic arrangements at different stages of prophase. Quantitative measurements confirmed that SUN1/KASH5, microtubules, and dynein but not actin were necessary for RPMs and that defects in meiotic recombination and synapsis resulted in altered RPMs.
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影响因子：
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作者：
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