Epidemiology and genotyping of Anaplasma marginale and co-infection with piroplasms and other Anaplasmataceae in cattle and buffaloes from Egypt.
Epidemiology and genotyping of Anaplasma marginale and co-infection with piroplasms and other Anaplasmataceae in cattle and buffaloes from Egypt.
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DOI：
10.1186/s13071-020-04372-z
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发表时间：
2020-09-29
影响因子：
3.2
通讯作者：
Silaghi C
中科院分区：
文献类型：
--
作者：
Al-Hosary A;Răileanu C;Tauchmann O;Fischer S;Nijhof AM;Silaghi C
边缘无原体是一种专性细胞内细菌，是热带和亚热带地区牛无原体病的主要病因。在埃及，缺乏关于边缘弧菌在反刍动物宿主中的流行率和流行基因型的数据。因此，本研究旨在(i)调查埃及牛和水牛中边缘蜱的存在、流行病学和基因型，（ii）评估合适的诊断工具，以及（iii）确定边缘蜱与其他选定的蜱传病原体的共感染。从埃及三个不同地区收集了394只动物（309头牛和85头水牛）的血液样本。比较了血涂片法、酶联免疫吸附法（ELISA）、PCR、实时荧光定量PCR和逆行免疫印迹法（RLB）检测边缘芽孢杆菌感染的敏感性和特异性。采用RLB法对边缘螺旋体、梨形螺旋体和其他无形体科螺旋体的共感染情况进行了调查，并对部分msp1α基因进行了扩增和测序，确定了边缘螺旋体的基因型。在68.3%的牛和29.4%的水牛中扩增出边缘无原体DNA。牛和水牛感染边缘芽孢杆菌的比例分别为50.2%和42.5%。血液涂片分析在16.2%的牛和2.4%的水牛中检测到该制剂。ELISA检测结果显示54.9%的牛有特异抗体。在牛和水牛中，边缘性无形体与几种蜱传病原体（环孢杆菌、牛巴贝虫、双生巴贝虫、隐蔽巴贝虫和高原无形体）有关。牛中边缘螺旋体感染率差异有统计学意义（P = 0.002281），其中3 ~ 5岁的感染率为79.2%，高于5岁以上（36.4%）、3岁以下（59.7%）和1岁（64.5%）。微卫星分析鉴定出15种不同的基因型。流行病学调查结果显示，在所有调查地区的牛和水牛中，边缘棘球蚴的流行率很高。观察到不同基因型的循环，其中大多数边缘棘球蚴基因型是埃及特有的。qPCR检测是检测牛和水牛中边缘螺旋体最敏感的工具，即使是在携带状态下，也强调了使用合适的诊断测试的重要性。
Anaplasma marginale is an obligate intracellular bacterium and the main cause of bovine anaplasmosis in tropical and subtropical regions. In Egypt, data regarding the prevalence of A. marginale in ruminant hosts and of the circulating genotypes is lacking. This study therefore aimed to (i) investigate the presence, epidemiology and genotypes of A. marginale in cattle and buffaloes in Egypt, (ii) to evaluate suitable diagnostic tools and (iii) to identify co-infections of A. marginale with other selected tick-borne pathogens. Blood samples were collected from 394 animals (309 cattle and 85 buffaloes) from three different areas in Egypt. For the detection of A. marginale infection, several tests were compared for their sensitivity and specificity: blood smear analysis, enzyme-linked immunosorbent assay (ELISA), PCR, real-time PCR and reverse line blot (RLB) assay. Co-infections with A. marginale, piroplasms and other Anaplasmataceae were surveyed by RLB while A. marginale genotypes were identified by amplifying and sequencing the partial msp1α gene. Anaplasma marginale DNA was amplified by qPCR in 68.3% of cattle and 29.4% of buffaloes. RLB showed infection with A. marginale in 50.2% of cattle and 42.5% of buffaloes. Blood smear analysis detected this agent in 16.2% of cattle and 2.4% of buffaloes. ELISA showed specific antibodies against A. marginale in 54.9% of cattle. Anaplasma marginale was associated, in cattle and buffaloes, with several tick-borne pathogens (Theileria annulata, Babesia bovis, Babesia bigemina, Babesia occultans and Anaplasma platys). A significant difference of A. marginale infection level was noticed in cattle, where animals between 3–5-years-old had a higher prevalence (79.2%) compared to those older than 5 years (36.4%) and younger than 3 years (59.7%) and one year (64.5%), respectively (P = 0.002281). Microsatellite analysis identified 15 different genotypes. The epidemiological findings revealed high prevalence of A. marginale in cattle and buffaloes in all the investigated areas. The circulation of diverse genotypes was observed, most of these A. marginale genotypes being specific for Egypt. The qPCR assay was confirmed to be the most sensitive tool for detection of A. marginale in cattle and buffaloes even in the carrier state, highlighting the importance of using suitable diagnostic tests.
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影响因子：
2.6
作者：
Alamzan, Consuelo;Medrano, Citlaly;de la Fuente, Jose
通讯作者：
de la Fuente, Jose
影响因子：
6.4
作者：
HARVEY, JW;SIMPSON, CF;GASKIN, JM
通讯作者：
GASKIN, JM
影响因子：
--
作者：
AL-Hosary, Amira A.T.
通讯作者：
AL-Hosary, Amira A.T.
影响因子：
9.4
作者：
ERIKS, IS;PALMER, GH;BARBET, AF
通讯作者：
BARBET, AF
影响因子：
3.7
作者：
Hailemariam Z;Krücken J;Baumann M;Ahmed JS;Clausen PH;Nijhof AM
通讯作者：
Nijhof AM